Molecular and epidemiologic characteristics of linezolid-resistant coagulase-negative staphylococci at a tertiary care hospital

Klaudia Kosowska-Shick; Kathleen G. Julian; Pamela L. McGhee; Peter C. Appelbaum; Cynthia J. Whitener

doi:10.1016/j.diagmicrobio.2010.05.007

Molecular and epidemiologic characteristics of linezolid-resistant coagulase-negative staphylococci at a tertiary care hospital

Klaudia Kosowska-Shick
, Kathleen G. Julian
, Pamela L. McGhee
, Peter C. Appelbaum
, Cynthia J. Whitener

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We investigated emergence of linezolid resistance among coagulase-negative staphylococci at our tertiary care center in 2007. All 17 cases were healthcare associated, and prior administration of linezolid was documented ≤2 months before first isolation of linezolid-resistant coagulase-negative staphylococci for all but 1 patient. Pulse-field gel electrophoresis analysis of the 14 available strains demonstrated 1 predominant clonal type, suggesting nosocomial spread. In addition to mutations in 23S rRNA and L4 previously described, we observed novel alterations in the 23S rRNA gene (G₂₂₁₅A) and in the L3 protein (substitutions L₁₀₁V, H₁₄₆Q/R, F₁₄₇I, V₁₅₄L, M₁₅₆T). The increase in linezolid-resistant coagulase-negative staphylococci correlated with nosocomial transmission of selected mutated strains in patients who had received linezolid.

Original language	English (US)
Pages (from-to)	34-39
Number of pages	6
Journal	Diagnostic Microbiology and Infectious Disease
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2010.05.007
State	Published - Sep 2010

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2010.05.007

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title = "Molecular and epidemiologic characteristics of linezolid-resistant coagulase-negative staphylococci at a tertiary care hospital",

abstract = "We investigated emergence of linezolid resistance among coagulase-negative staphylococci at our tertiary care center in 2007. All 17 cases were healthcare associated, and prior administration of linezolid was documented ≤2 months before first isolation of linezolid-resistant coagulase-negative staphylococci for all but 1 patient. Pulse-field gel electrophoresis analysis of the 14 available strains demonstrated 1 predominant clonal type, suggesting nosocomial spread. In addition to mutations in 23S rRNA and L4 previously described, we observed novel alterations in the 23S rRNA gene (G2215A) and in the L3 protein (substitutions L101V, H146Q/R, F147I, V154L, M156T). The increase in linezolid-resistant coagulase-negative staphylococci correlated with nosocomial transmission of selected mutated strains in patients who had received linezolid.",

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AU - Kosowska-Shick, Klaudia

AU - Julian, Kathleen G.

AU - McGhee, Pamela L.

AU - Appelbaum, Peter C.

AU - Whitener, Cynthia J.

PY - 2010/9

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N2 - We investigated emergence of linezolid resistance among coagulase-negative staphylococci at our tertiary care center in 2007. All 17 cases were healthcare associated, and prior administration of linezolid was documented ≤2 months before first isolation of linezolid-resistant coagulase-negative staphylococci for all but 1 patient. Pulse-field gel electrophoresis analysis of the 14 available strains demonstrated 1 predominant clonal type, suggesting nosocomial spread. In addition to mutations in 23S rRNA and L4 previously described, we observed novel alterations in the 23S rRNA gene (G2215A) and in the L3 protein (substitutions L101V, H146Q/R, F147I, V154L, M156T). The increase in linezolid-resistant coagulase-negative staphylococci correlated with nosocomial transmission of selected mutated strains in patients who had received linezolid.

AB - We investigated emergence of linezolid resistance among coagulase-negative staphylococci at our tertiary care center in 2007. All 17 cases were healthcare associated, and prior administration of linezolid was documented ≤2 months before first isolation of linezolid-resistant coagulase-negative staphylococci for all but 1 patient. Pulse-field gel electrophoresis analysis of the 14 available strains demonstrated 1 predominant clonal type, suggesting nosocomial spread. In addition to mutations in 23S rRNA and L4 previously described, we observed novel alterations in the 23S rRNA gene (G2215A) and in the L3 protein (substitutions L101V, H146Q/R, F147I, V154L, M156T). The increase in linezolid-resistant coagulase-negative staphylococci correlated with nosocomial transmission of selected mutated strains in patients who had received linezolid.

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Molecular and epidemiologic characteristics of linezolid-resistant coagulase-negative staphylococci at a tertiary care hospital

Abstract

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