TY - CHAP
T1 - Molecular cell biology of male meiotic chromosomes and isolation of male meiocytes in Arabidopsis thaliana
AU - Wang, Yingxiang
AU - Cheng, Zhihao
AU - Lu, Pingli
AU - Timofejeva, Ljudmilla
AU - Ma, Hong
PY - 2014
Y1 - 2014
N2 - Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.
AB - Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.
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U2 - 10.1007/978-1-4614-9408-9-10
DO - 10.1007/978-1-4614-9408-9-10
M3 - Chapter
C2 - 24395259
SN - 9781461494072
VL - 1110
T3 - Methods in Molecular Biology
SP - 217
EP - 230
BT - Methods in Molecular Biology
ER -