Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus

R. H. Ffrench-Constant; D. P. Mortlock; C. D. Shaffer; R. J. Macintyre; R. T. Roush

Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus

R. H. Ffrench-Constant, D. P. Mortlock, C. D. Shaffer, R. J. Macintyre, R. T. Roush

College of Agricultural Sciences

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280 Scopus citations

Abstract

Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the γ-aminobutyric acid subtype A (GABA_A) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a "chromosomal walk" and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABA_A subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.

Original language	English (US)
Pages (from-to)	7209-7213
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	16
State	Published - 1991

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title = "Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus",

abstract = "Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the γ-aminobutyric acid subtype A (GABAA) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a {"}chromosomal walk{"} and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABAA subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.",

author = "Ffrench-Constant, {R. H.} and Mortlock, {D. P.} and Shaffer, {C. D.} and Macintyre, {R. J.} and Roush, {R. T.}",

year = "1991",

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Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus. / Ffrench-Constant, R. H.; Mortlock, D. P.; Shaffer, C. D. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 16, 1991, p. 7209-7213.

Research output: Contribution to journal › Article › peer-review

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T1 - Molecular cloning and transformation of cyclodiene resistance in Drosophila

T2 - An invertebrate γ-aminobutyric acid subtype A receptor locus

AU - Ffrench-Constant, R. H.

AU - Mortlock, D. P.

AU - Shaffer, C. D.

AU - Macintyre, R. J.

AU - Roush, R. T.

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AB - Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the γ-aminobutyric acid subtype A (GABAA) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a "chromosomal walk" and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABAA subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.

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Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus

Abstract

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