@article{7926f6dc84df42e28583638c1789c5c0,
title = "Molecular cloning, expression, and activity of zebrafish semaphorin Z1a",
abstract = "Semaphorins/collapsins are a large family of secreted and cell surface molecules that are thought to guide growth cones to their targets. Although some members are clearly repulsive to specific growth cones in vitro, the in vivo role of many of these molecules in vertebrate embryos is still unclear. As a first step towards clarifying the in vivo role of semaphorins/collapsins, we analyzed semaZ1a in the simple and well- characterized zebrafish embryo. SemaZ1a is a secreted molecule that is highly homologous to Sema III/D/collapsin-1, and it can collapse chick dorsal root ganglion growth cones in vitro. It is expressed in highly specific patterns within the developing embryo, which suggests that it influences outgrowth by a variety of growth cones including those of the posterior lateral line ganglion. Consistent with this hypothesis, the peripherally extending growth cones of posterior lateral line neurons retract and partially collapse during normal outgrowth.",
author = "Yee, {C. S.} and A. Chandrasekhar and Halloran, {M. C.} and W. Shoji and Warren, {J. T.} and Kuwada, {J. Y.}",
note = "Funding Information: We thank Drs. J. Lauderdale and N. Brown for many helpful discussions during the course of our experiments. Thanks are due to Drs. Roos, Bernhardt, and Schachner for sharing unpublished results; Dr. J. Raper for the chick collapsin-1 cDNA; and Drs. K. Zinn and H. Okamoto for zebrafish cDNA libraries. Special thanks are due to Dr. Fengyun Su for supervision of our zebrafish breeding colony. Molecular analysis was in part supported by an NIH grant (M01RR00042) to the General Clinical Research Center at the University of Michigan. The research was supported by an NRSA postdoctoral fellowship to M.C.H., by a postdoctoral fellowship from the Center for Organogenesis at the University of Michigan to A.C, by a long-term postdoctoral fellowship to W.S from the Human Frontier Science Program Organization, by a postdoctoral fellowship from the Spinal Cord Research Foundation/Paralyzed Veterans of America to J.T.W., and by grants from the NINDS (NS24848) and APA (KAI-9603 and KAR1-9704) to J.Y.K.",
year = "1999",
month = apr,
doi = "10.1016/S0361-9230(99)00038-6",
language = "English (US)",
volume = "48",
pages = "581--593",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "6",
}