TY - JOUR
T1 - Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain
AU - Nagamine, Kentaro
AU - Kudoh, Jun
AU - Minoshima, Shinsei
AU - Kawasaki, Kazuhiko
AU - Asakawa, Shuichi
AU - Ito, Fumiaki
AU - Shimizu, Nobuyoshi
N1 - Funding Information:
The authors thank Drs. E. Nakato and K. Shibuya and all the members of the sequencing team in the Laboratory of Genomic Medicine for their contribution to this work, Drs. H. Ichikawa and M. Ohki (National Cancer Center Research Institute, Tokyo, Japan) for the NotI linking clone, and Drs. J. Hazan and J. Weissenbach (Géné-thon, Evry, France) for the D21S154 marker (IP21K443). This work was supported in part by Grants in Aid for Scientific Research on Priority Areas and Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan and the Fund for Human Genome Sequencing Project from the Japan Science and Technology Corporation (JST) and by the “Research for the Future” Program from the Japan Society for the Promotion of Science (JSPS).
PY - 1998/11/15
Y1 - 1998/11/15
N2 - We have isolated cDNA clones for a novel human protein, TRPC7 (transient receptor potential-related channels), which consists of 1503 amino acid residues from the fetal brain and caudate nucleus cDNA libraries. Northern blot analysis indicated that the TRPC7 gene is highly expressed as a 6.5-kb transcript in brain. The TRPC7 protein has significant homology with Caenorhabditis elegans hypothetical proteins T01H8.5, C05C12.3, and F54D1.5 and with Drosophila and human transient receptor potential (trp) proteins. The TRPC7 protein has seven putative transmembrane domains that probably constitute a Ca2+ channel as in the above-mentioned proteins. Genomic sequencing revealed that the TRPC7 gene consists of 32 exons spanning approximately 90 kb. The TRPC7 gene was mapped between D21S400 and D21S171 on human chromosome 21q22.3, 14 kb distal to a NotI site in D21S400. This novel TRPC7 gene could be a candidate gene for genetic disorders such as bipolar affective disorder, nonsyndromic hereditary deafness, Knobloch syndrome, and holoprosencephaly, which were mapped to this region.
AB - We have isolated cDNA clones for a novel human protein, TRPC7 (transient receptor potential-related channels), which consists of 1503 amino acid residues from the fetal brain and caudate nucleus cDNA libraries. Northern blot analysis indicated that the TRPC7 gene is highly expressed as a 6.5-kb transcript in brain. The TRPC7 protein has significant homology with Caenorhabditis elegans hypothetical proteins T01H8.5, C05C12.3, and F54D1.5 and with Drosophila and human transient receptor potential (trp) proteins. The TRPC7 protein has seven putative transmembrane domains that probably constitute a Ca2+ channel as in the above-mentioned proteins. Genomic sequencing revealed that the TRPC7 gene consists of 32 exons spanning approximately 90 kb. The TRPC7 gene was mapped between D21S400 and D21S171 on human chromosome 21q22.3, 14 kb distal to a NotI site in D21S400. This novel TRPC7 gene could be a candidate gene for genetic disorders such as bipolar affective disorder, nonsyndromic hereditary deafness, Knobloch syndrome, and holoprosencephaly, which were mapped to this region.
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U2 - 10.1006/geno.1998.5551
DO - 10.1006/geno.1998.5551
M3 - Article
C2 - 9806837
AN - SCOPUS:0032533315
SN - 0888-7543
VL - 54
SP - 124
EP - 131
JO - Genomics
JF - Genomics
IS - 1
ER -