Molecular markers as a method to evaluate the movement of Hypothenemus hampei (Ferrari)

Zulma Nancy Gil, Pablo Benavides, Og De Souza, Flor Edith Acevedo, Eraldo Lima, Yong Zhang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The objective of this research was to develop a methodology to describe the movement of the coffee berry borer Hypothenemus hampei (Coleoptera: Curculionidae) in the field through: (i) the evaluation of allele variation of a microsatellite marker on polymorphic Colombian H. hampei populations; (ii) the invention of a device for releasing H. hampei adults; (iii) the standardization of a release-recapture technique for H. hampei populations; (iv) the estimation of the flight distance of the insect; and (v) the calculation of a mathematical expression that describes the movement of H. hampei in space over time. The results indicated that: (i) the microsatellite molecular marker HHK.1.6 was exclusively present in a population from Guapotá-Santander, was dominant and allows the evaluation of H. hampei movement for several generations; (ii) a device that released 88.8% of H. hampei adults in 2 s was designed; (iii) this device was used as H. hampei populations containing HHK.1.6 marker release strategy, and coffee seeds as recapture strategy; (iv) it was estimated that H. hampei adults flew as far as 65 m, however, 90% were recovered in a radius of <40m. Finally, (v) the mathematical expression that described the movement of H. hampei in space over time was Ŷ = αβX′, being Ŷ the average number of borer beetles recaptured per tree, and x the distance in meters. This method will allow to determine the movement of H. hampei from different environmental and ecological scenarios.

Original languageEnglish (US)
JournalJournal of Insect Science
Issue number1
StatePublished - 2015

All Science Journal Classification (ASJC) codes

  • Insect Science


Dive into the research topics of 'Molecular markers as a method to evaluate the movement of Hypothenemus hampei (Ferrari)'. Together they form a unique fingerprint.

Cite this