Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

J. R. Petrulis; G. H. Perdew

doi:10.2144/01314st02

Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

J. R. Petrulis, G. H. Perdew

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.

Original language	English (US)
Pages (from-to)	772-775
Number of pages	4
Journal	BioTechniques
Volume	31
Issue number	4
DOIs	https://doi.org/10.2144/01314st02
State	Published - 2001

All Science Journal Classification (ASJC) codes

Biotechnology
General Biochemistry, Genetics and Molecular Biology

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10.2144/01314st02

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title = "Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells",

abstract = "A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.",

author = "Petrulis, {J. R.} and Perdew, {G. H.}",

year = "2001",

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AB - A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time.

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Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells

Abstract

All Science Journal Classification (ASJC) codes

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