Monoclonal antibodies that recognize discrete forms of tubulin.

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Abstract

Anti-tubulin antibodies secreted by plasmacytoma NSI-spleen cell hybrids were detected by an indirect binding assay. Different antibodies bound to different combinations of the tubulins as resolved by isoelectric focusing. Two monoclonal antibodies (TUB 2.1 and TUB 2.5) labeled only (i) the tubulin band on a polyacrylamide electropherogram and (ii) beta-tubulins as resolved by isoelectric focusing. The fraction that was specifically bound and eluted from antibody affinity columns was enriched in beta-tubulins as compared with alpha-tubulins, suggesting the possibility of some soluble tubulin homodimers and alpha,beta-heterodimers. Double labeling experiments were used to show that all detectable microtubules contained beta-tubulin.

Original languageEnglish (US)
Pages (from-to)2579-2583
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number8
DOIs
StatePublished - Apr 1982

All Science Journal Classification (ASJC) codes

  • General

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