@article{1ee78d72138b46398eb252e73419b67a,
title = "Monoclonal antibodies to s and n sars-cov-2 proteins as probes to assess structural and antigenic properties of coronaviruses",
abstract = "Antibodies targeting the spike (S) and nucleocapsid (N) proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential tools. In addition to important roles in the treatment and diagnosis of infection, the availability of high-quality specific antibodies for the S and N proteins is essential to facilitate basic research of virus replication and in the characterization of mutations responsible for variants of concern. We have developed panels of mouse and rabbit monoclonal antibodies (mAbs) to the SARS-CoV-2 spike receptor-binding domain (S-RBD) and N protein for functional and antigenic analyses. The mAbs to the S-RBD were tested for neutralization of native SARS-CoV-2, with several exhibiting neutralizing activity. The panels of mAbs to the N protein were assessed for cross-reactivity with the SARS-CoV and Middle East respiratory syndrome (MERS)-CoV N proteins and could be subdivided into sets that showed unique specificity for SARS-CoV-2 N protein, cross-reactivity between SARS-CoV-2 and SARS-CoV N proteins only, or cross-reactivity to all three coronavirus N proteins tested. Partial mapping of N-reactive mAbs were conducted using truncated fragments of the SARS-CoV-2 N protein and revealed near complete coverage of the N protein. Collectively, these sets of mouse and rabbit monoclonal antibodies can be used to examine structure/function studies for N proteins and to define the surface location of virus neutralizing epitopes on the RBD of the S protein.",
author = "Rinki Kumar and Christensen, {Neil D.} and {Kaddis Maldonado}, {Rebecca J.} and Bewley, {Maria C.} and Alexandria Ostman and Malgorzata Sudol and Chen, {Eunice C.} and Buchkovich, {Natalie W.} and Abhinay Gontu and {Surendran Nair}, Meera and Nissly, {Ruth H.} and Minns, {Allen M.} and Vivek Kapur and Randall Rossi and Kuchipudi, {Suresh V.} and Lindner, {Scott E.} and Parent, {Leslie J.} and Flanagan, {John M.} and Buchkovich, {Nicholas J.}",
note = "Funding Information: Funding: This research was funded by the Pennsylvania Department of Community & Economic Development (N.J.B., L.J.P., J.M.F., M.C.B., N.D.C.), by a grant from the Department of Medicine at the Penn State College of Medicine (L.J.P., J.M.F.), and by seed funding from the Huck Institutes of the Life Sciences and Penn State University (S.E.L., S.V.K.). Funding Information: This research was funded by the Pennsylvania Department of Community & Economic Development (N.J.B., L.J.P., J.M.F., M.C.B., N.D.C.), by a grant from the Department of Medicine at the Penn State College of Medicine (L.J.P., J.M.F.), and by seed funding from the Huck Institutes of the Life Sciences and Penn State University (S.E.L., S.V.K.). We would like to acknowledge the contributions of Sarah Brendle from the Antibody Core at the Penn State College of Medicine, of which N.D.C. is the Director. We would like to thank Breanna Rice for technical contributions to the study. We would also like to acknowledge the Sartorius Huck Cell Culture Facility for early access for production of S-RBD. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = oct,
doi = "10.3390/v13101899",
language = "English (US)",
volume = "13",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "10",
}