TY - JOUR
T1 - Multi-Omics Strategies for Investigating the Microbiome in Toxicology Research
AU - Morgan, Ethan W.
AU - Perdew, Gary H.
AU - Patterson, Andrew D.
N1 - Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - Microbial communities on and within the host contact environmental pollutants, toxic compounds, and other xenobiotic compounds. These communities of bacteria, fungi, viruses, and archaea possess diverse metabolic potential to catabolize compounds and produce new metabolites. Microbes alter chemical disposition thus making the microbiome a natural subject of interest for toxicology. Sequencing and metabolomics technologies permit the study of microbiomes altered by acute or long-term exposure to xenobiotics. These investigations have already contributed to and are helping to re-interpret traditional understandings of toxicology. The purpose of this review is to provide a survey of the current methods used to characterize microbes within the context of toxicology. This will include discussion of commonly used techniques for conducting omic-based experiments, their respective strengths and deficiencies, and how forward-looking techniques may address present shortcomings. Finally, a perspective will be provided regarding common assumptions that currently impede microbiome studies from producing causal explanations of toxicologic mechanisms.
AB - Microbial communities on and within the host contact environmental pollutants, toxic compounds, and other xenobiotic compounds. These communities of bacteria, fungi, viruses, and archaea possess diverse metabolic potential to catabolize compounds and produce new metabolites. Microbes alter chemical disposition thus making the microbiome a natural subject of interest for toxicology. Sequencing and metabolomics technologies permit the study of microbiomes altered by acute or long-term exposure to xenobiotics. These investigations have already contributed to and are helping to re-interpret traditional understandings of toxicology. The purpose of this review is to provide a survey of the current methods used to characterize microbes within the context of toxicology. This will include discussion of commonly used techniques for conducting omic-based experiments, their respective strengths and deficiencies, and how forward-looking techniques may address present shortcomings. Finally, a perspective will be provided regarding common assumptions that currently impede microbiome studies from producing causal explanations of toxicologic mechanisms.
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U2 - 10.1093/toxsci/kfac029
DO - 10.1093/toxsci/kfac029
M3 - Review article
C2 - 35285497
AN - SCOPUS:85131224449
SN - 1096-6080
VL - 187
SP - 189
EP - 213
JO - Toxicological Sciences
JF - Toxicological Sciences
IS - 2
ER -