Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum

Antonio Esaú Del Río Castillo, Antonio De León-Rodriguez, Mauricio Terrones, Ana Paulina Barba de la Rosa

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.

Original languageEnglish (US)
Pages (from-to)902-909
Number of pages8
StatePublished - Oct 30 2021

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Materials Science


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