TY - JOUR
T1 - Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum
AU - Del Río Castillo, Antonio Esaú
AU - De León-Rodriguez, Antonio
AU - Terrones, Mauricio
AU - Barba de la Rosa, Ana Paulina
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10/30
Y1 - 2021/10/30
N2 - Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.
AB - Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.
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U2 - 10.1016/j.carbon.2021.08.052
DO - 10.1016/j.carbon.2021.08.052
M3 - Article
AN - SCOPUS:85114814013
SN - 0008-6223
VL - 184
SP - 902
EP - 909
JO - Carbon
JF - Carbon
ER -