Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum

Antonio Esaú Del Río Castillo; Antonio De León-Rodriguez; Mauricio Terrones; Ana Paulina Barba de la Rosa

doi:10.1016/j.carbon.2021.08.052

Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum

Antonio Esaú Del Río Castillo
, Antonio De León-Rodriguez
, Mauricio Terrones
, Ana Paulina Barba de la Rosa

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 10⁷ to 2.1 × 10⁷ cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.

Original language	English (US)
Pages (from-to)	902-909
Number of pages	8
Journal	Carbon
Volume	184
DOIs	https://doi.org/10.1016/j.carbon.2021.08.052
State	Published - Oct 30 2021

All Science Journal Classification (ASJC) codes

General Chemistry
General Materials Science

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10.1016/j.carbon.2021.08.052

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title = "Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum",

abstract = "Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.",

author = "\{Del R{\'i}o Castillo\}, \{Antonio Esa{\'u}\} and \{De Le{\'o}n-Rodriguez\}, Antonio and Mauricio Terrones and \{Barba de la Rosa\}, \{Ana Paulina\}",

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doi = "10.1016/j.carbon.2021.08.052",

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AU - Del Río Castillo, Antonio Esaú

AU - De León-Rodriguez, Antonio

AU - Terrones, Mauricio

AU - Barba de la Rosa, Ana Paulina

PY - 2021/10/30

Y1 - 2021/10/30

N2 - Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.

AB - Genetically transformed Bifidobacterium longum, a probiotic bacterium in the human intestines, has a high potential for in-situ delivery of therapeutic proteins. However, one limitation is its low transformation efficiency caused by its thick membrane as it is a Gram-positive bacterium. Here we present an excellent transformation efficiency of B. longum (up to 1 × 107 to 2.1 × 107 cells/μg of DNA), obtained when oxidized multi-walled carbon nanotubes are used in synergy with classic transformation methods. This approach opens a fruitful avenue for B. longum transformations that could increase its potential for in-situ delivery systems of recombinant proteins.

UR - https://www.scopus.com/pages/publications/85114814013

UR - https://www.scopus.com/pages/publications/85114814013#tab=citedBy

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DO - 10.1016/j.carbon.2021.08.052

M3 - Article

AN - SCOPUS:85114814013

SN - 0008-6223

VL - 184

SP - 902

EP - 909

JO - Carbon

JF - Carbon

ER -

Multi-walled carbon nanotubes enhance the genetic transformation of Bifidobacterium longum

Abstract

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