TY - JOUR
T1 - Multiple Separations in Microfabricated Channels
T2 - From Biological Microenvironments to DNA
AU - Bullard, Katherine M.
AU - Hietpas, Paula Beyer
AU - Ewing, Andrew G.
N1 - Funding Information:
Support for this work from the National Science Foundation and the National Institutes of Health is gratefully acknowledged.
PY - 1998
Y1 - 1998
N2 - A continuous sample introduction and separation scheme is presented as an alternative to the current slab gel and microfabricated chip technologies for biological separations. This new technique involves continuous sample introduction via a conventional small bore capillary coupled to a small dimension rectangular channel. Both free zone and size based separations have been carried out in the rectangular channel. Laser induced fluorescence and electrochemical detection schemes have been employed with this technique. Some of the areas this technology has been used to investigate include monitoring dynamic events from microenvironments, monitoring analytes over long periods of time, and performing DNA separations.
AB - A continuous sample introduction and separation scheme is presented as an alternative to the current slab gel and microfabricated chip technologies for biological separations. This new technique involves continuous sample introduction via a conventional small bore capillary coupled to a small dimension rectangular channel. Both free zone and size based separations have been carried out in the rectangular channel. Laser induced fluorescence and electrochemical detection schemes have been employed with this technique. Some of the areas this technology has been used to investigate include monitoring dynamic events from microenvironments, monitoring analytes over long periods of time, and performing DNA separations.
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U2 - 10.1023/A:1009926205209
DO - 10.1023/A:1009926205209
M3 - Article
AN - SCOPUS:0002511496
SN - 1387-2176
VL - 1
SP - 27
EP - 37
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 1
ER -