TY - JOUR
T1 - Myosin chains of myocardial tissue - I. Purification and immunological properties of myosin heavy chains
AU - Wikman-Coffelt, Joan
AU - Zelis, Robert
AU - Fenner, Claudia
AU - Mason, Dean T.
PY - 1973/4/16
Y1 - 1973/4/16
N2 - An antiserum specific to dog myocardial myosin has been developed against highly purified myosin heavy chains. The antiserum is specific for the heavy chains of myosin, giving a single precipitin line in an immunodiffusion assay for either the heavy chains of myosin or native myosin, and does not react with any other myocardial proteins. In such assays myosin acts as a single, uniform antigen. Using this antiserum, a radioimmunoassay has been developed to quantitate myosin in a homogenate of myocardial tissue containing free myosin dissociated from other cellular components.
AB - An antiserum specific to dog myocardial myosin has been developed against highly purified myosin heavy chains. The antiserum is specific for the heavy chains of myosin, giving a single precipitin line in an immunodiffusion assay for either the heavy chains of myosin or native myosin, and does not react with any other myocardial proteins. In such assays myosin acts as a single, uniform antigen. Using this antiserum, a radioimmunoassay has been developed to quantitate myosin in a homogenate of myocardial tissue containing free myosin dissociated from other cellular components.
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U2 - 10.1016/0006-291X(73)90040-5
DO - 10.1016/0006-291X(73)90040-5
M3 - Article
C2 - 4634071
AN - SCOPUS:0015927320
SN - 0006-291X
VL - 51
SP - 1097
EP - 1104
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -