Abstract
An antiserum specific to dog myocardial myosin has been developed against highly purified myosin heavy chains. The antiserum is specific for the heavy chains of myosin, giving a single precipitin line in an immunodiffusion assay for either the heavy chains of myosin or native myosin, and does not react with any other myocardial proteins. In such assays myosin acts as a single, uniform antigen. Using this antiserum, a radioimmunoassay has been developed to quantitate myosin in a homogenate of myocardial tissue containing free myosin dissociated from other cellular components.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1097-1104 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 51 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 16 1973 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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