N(τ)-methylhistidine release: Contributions of rat skeletal muscle, GI tract, and skin

S. J. Wassner, J. B. Li

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The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N(τ)-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol.h-1.100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol.h-1.100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol.h-1.100 g body wt-1 or approximately 41% of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8% of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4%, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24%/day versus 1.4%/day for skeletal muscle actomyosin.

Original languageEnglish (US)
Pages (from-to)E293-E297
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number4
StatePublished - 1982

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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