TY - JOUR
T1 - Nanoindentation of Isometric Viruses on Deterministically Corrugated Substrates
AU - Hernando-Pérez, M.
AU - Zeng, C.
AU - Delalande, L.
AU - Tsvetkova, I. B.
AU - Bousquet, A.
AU - Tayachi-Pigeonnat, M.
AU - Temam, R.
AU - Dragnea, B.
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2016/1/21
Y1 - 2016/1/21
N2 - It has been just over 100 years since inventor Joseph Coyle perfected the egg carton-a package format that has known very little changes since its first appearance (Dhillon, S. B. C. Inventor Created Better Way to Carry Eggs. In The Globe and Mail Vancouver, 2013). In this article, we extend Coyles old idea to the study of mechanical properties of viruses. Virus stiffness, strength, and breaking force obtained by force spectroscopy atomic force microscopy (AFM) provide the knowledge required for designing nanocontainers for applications in biotechnology and medicine, and for understanding the fundamentals of virus-host interaction such as virus translocation from one cellular compartment to another. In previous studies, virus particles adsorbed on flat surfaces from a physiological buffer were subjected to directional deformation by a known force exerted via a microscopic probe. The affinity between the virus shell and surface is required to be strong enough to anchor particles on the substrate while they are indented or imaged, yet sufficiently weak to preserve the native structure and interactions prior deformation. The specific question addressed here is whether an experimental scheme characterized by increased contact area and stable mechanical equilibrium under directional compression would provide a more reliable characterization than the traditional flat substrate approach.
AB - It has been just over 100 years since inventor Joseph Coyle perfected the egg carton-a package format that has known very little changes since its first appearance (Dhillon, S. B. C. Inventor Created Better Way to Carry Eggs. In The Globe and Mail Vancouver, 2013). In this article, we extend Coyles old idea to the study of mechanical properties of viruses. Virus stiffness, strength, and breaking force obtained by force spectroscopy atomic force microscopy (AFM) provide the knowledge required for designing nanocontainers for applications in biotechnology and medicine, and for understanding the fundamentals of virus-host interaction such as virus translocation from one cellular compartment to another. In previous studies, virus particles adsorbed on flat surfaces from a physiological buffer were subjected to directional deformation by a known force exerted via a microscopic probe. The affinity between the virus shell and surface is required to be strong enough to anchor particles on the substrate while they are indented or imaged, yet sufficiently weak to preserve the native structure and interactions prior deformation. The specific question addressed here is whether an experimental scheme characterized by increased contact area and stable mechanical equilibrium under directional compression would provide a more reliable characterization than the traditional flat substrate approach.
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U2 - 10.1021/acs.jpcb.5b08362
DO - 10.1021/acs.jpcb.5b08362
M3 - Article
C2 - 26674071
AN - SCOPUS:84955484435
SN - 1520-6106
VL - 120
SP - 340
EP - 347
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 2
ER -