Nanoparticle-based protein detection by optical shift of a resonant microcavity

Miguel A. Santiago-Cordoba; Svetlana V. Boriskina; Frank Vollmer; Melik C. Demirel

doi:10.1063/1.3599706

Nanoparticle-based protein detection by optical shift of a resonant microcavity

Miguel A. Santiago-Cordoba, Svetlana V. Boriskina, Frank Vollmer, Melik C. Demirel

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164 Scopus citations

Abstract

We demonstrated a biosensing approach which, for the first time, combines the high sensitivity of whispering gallery modes (WGMs) with a metallic nanoparticle-based assay. We provided a computational model based on generalized Mie theory to explain the higher sensitivity of protein detection. We quantitatively analyzed the binding of a model protein (i.e., Bovine Serum Albumin) to gold nanoparticles from high-Q WGM resonance frequency shifts, and fit the results to an adsorption isotherm, which agrees with the theoretical predictions of a two-component adsorption model.

Original language	English (US)
Article number	073701
Journal	Applied Physics Letters
Volume	99
Issue number	7
DOIs	https://doi.org/10.1063/1.3599706
State	Published - Aug 15 2011

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.3599706

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title = "Nanoparticle-based protein detection by optical shift of a resonant microcavity",

abstract = "We demonstrated a biosensing approach which, for the first time, combines the high sensitivity of whispering gallery modes (WGMs) with a metallic nanoparticle-based assay. We provided a computational model based on generalized Mie theory to explain the higher sensitivity of protein detection. We quantitatively analyzed the binding of a model protein (i.e., Bovine Serum Albumin) to gold nanoparticles from high-Q WGM resonance frequency shifts, and fit the results to an adsorption isotherm, which agrees with the theoretical predictions of a two-component adsorption model.",

author = "Santiago-Cordoba, {Miguel A.} and Boriskina, {Svetlana V.} and Frank Vollmer and Demirel, {Melik C.}",

note = "Funding Information: We gratefully acknowledge financial support for this work from the Wyss Institute at Harvard, as well as the Rowland institute at Harvard for the cooperation in the use of their facilities. ",

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AU - Boriskina, Svetlana V.

AU - Vollmer, Frank

AU - Demirel, Melik C.

N1 - Funding Information: We gratefully acknowledge financial support for this work from the Wyss Institute at Harvard, as well as the Rowland institute at Harvard for the cooperation in the use of their facilities.

PY - 2011/8/15

Y1 - 2011/8/15

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AB - We demonstrated a biosensing approach which, for the first time, combines the high sensitivity of whispering gallery modes (WGMs) with a metallic nanoparticle-based assay. We provided a computational model based on generalized Mie theory to explain the higher sensitivity of protein detection. We quantitatively analyzed the binding of a model protein (i.e., Bovine Serum Albumin) to gold nanoparticles from high-Q WGM resonance frequency shifts, and fit the results to an adsorption isotherm, which agrees with the theoretical predictions of a two-component adsorption model.

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Nanoparticle-based protein detection by optical shift of a resonant microcavity

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