Nanopore Digital Counting of Amplicons for Ultrasensitive Electronic DNA Detection

Zifan Tang, Gihoon Choi, Reza Nouri, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the feasibility of using the single molecule sensing nanopore as a digital counter to enumerate the amplicons for ultrasensitive electronic nucleic acid analysis. We show that the nanopore digital counting approach could capture the DNA replication dynamics in the LAMP reaction and has the potential to be used in a qualitative test as well as in a quantitative test. By keeping nanopores as simple as possible and by leveraging the amplification assay's high sensitivity, the amplification-coupled nanopore digital counting approach provides a promising optics-free method for highly sensitive and specific nucleic acid testing.

Original languageEnglish (US)
Title of host publication2019 IEEE International Electron Devices Meeting, IEDM 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728140315
DOIs
StatePublished - Dec 2019
Event65th Annual IEEE International Electron Devices Meeting, IEDM 2019 - San Francisco, United States
Duration: Dec 7 2019Dec 11 2019

Publication series

NameTechnical Digest - International Electron Devices Meeting, IEDM
Volume2019-December
ISSN (Print)0163-1918

Conference

Conference65th Annual IEEE International Electron Devices Meeting, IEDM 2019
Country/TerritoryUnited States
CitySan Francisco
Period12/7/1912/11/19

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Materials Chemistry

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