TY - JOUR
T1 - Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells
AU - Zhou, Jing
AU - Xu, Ren He
AU - Wang, Yong
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-ß secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.
AB - Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-ß secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.
UR - http://www.scopus.com/inward/record.url?scp=79953277201&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953277201&partnerID=8YFLogxK
U2 - 10.1504/IJFIPM.2009.022838
DO - 10.1504/IJFIPM.2009.022838
M3 - Article
AN - SCOPUS:79953277201
SN - 1756-2104
VL - 2
SP - 77
EP - 88
JO - International Journal of Functional Informatics and Personalised Medicine
JF - International Journal of Functional Informatics and Personalised Medicine
IS - 1
ER -