TY - JOUR
T1 - Nanoscale Amperometry Reveals that Only a Fraction of Vesicular Serotonin Content is Released During Exocytosis from Beta Cells
AU - Hatamie, Amir
AU - Ren, Lin
AU - Dou, Haiqiang
AU - Gandasi, Nikhil R.
AU - Rorsman, Patrik
AU - Ewing, Andrew
N1 - Publisher Copyright:
© 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH
PY - 2021/3/29
Y1 - 2021/3/29
N2 - Recent work has shown that chemical release during the fundamental cellular process of exocytosis in model cell lines is not all-or-none. We tested this theory for vesicular release from single pancreatic beta cells. The vesicles in these cells release insulin, but also serotonin, which is detectible with amperometric methods. Traditionally, it is assumed that exocytosis in beta cells is all-or-none. Here, we use a multidisciplinary approach involving nanoscale amperometric chemical methods to explore the chemical nature of insulin exocytosis. We amperometrically quantified the number of serotonin molecules stored inside of individual nanoscale vesicles (39 317±1611) in the cell cytoplasm before exocytosis and the number of serotonin molecules released from single cells (13 310±1127) for each stimulated exocytosis event. Thus, beta cells release only one-third of their granule content, clearly supporting partial release in this system. We discuss these observations in the context of type-2 diabetes.
AB - Recent work has shown that chemical release during the fundamental cellular process of exocytosis in model cell lines is not all-or-none. We tested this theory for vesicular release from single pancreatic beta cells. The vesicles in these cells release insulin, but also serotonin, which is detectible with amperometric methods. Traditionally, it is assumed that exocytosis in beta cells is all-or-none. Here, we use a multidisciplinary approach involving nanoscale amperometric chemical methods to explore the chemical nature of insulin exocytosis. We amperometrically quantified the number of serotonin molecules stored inside of individual nanoscale vesicles (39 317±1611) in the cell cytoplasm before exocytosis and the number of serotonin molecules released from single cells (13 310±1127) for each stimulated exocytosis event. Thus, beta cells release only one-third of their granule content, clearly supporting partial release in this system. We discuss these observations in the context of type-2 diabetes.
UR - http://www.scopus.com/inward/record.url?scp=85101629109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85101629109&partnerID=8YFLogxK
U2 - 10.1002/anie.202015902
DO - 10.1002/anie.202015902
M3 - Article
C2 - 33340209
AN - SCOPUS:85101629109
SN - 1433-7851
VL - 60
SP - 7593
EP - 7596
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 14
ER -