TY - JOUR
T1 - Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand
AU - Waitayakul, Amornrat
AU - Somsri, Sangdao
AU - Sattabongkot, Jetsumon
AU - Looareesuwan, Sornchai
AU - Cui, Liwang
AU - Udomsangpetch, Rachanee
N1 - Funding Information:
We would like to thank Dr. Wej Choochote from Faculty of Medicine, Chaingmai University, for providing additional Anopheles species. We thank NIH Fogarty International Center for support (D43 TW006571) to L. Cui and R. Udomsangpetch. AW is supported by the Faculty of Graduate Studies, Mahidol University.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/4
Y1 - 2006/4
N2 - During blood feeding, arthropod vectors inject saliva into vertebrate hosts. The saliva is biochemically complex and pharmacologically active, and may play an important role in pathogen transmission. To examine whether mosquito saliva could elicit humoral immune response in humans under natural conditions, we have collected sera from malaria patients, healthy villagers, and people from a non-malarious region in Thailand. Here we have demonstrated that anti-Anopheles salivary protein antibodies occurred predominantly in patients with acute Plasmodium falciparum or P. vivax malaria, whereas people from a non-malarious area had no such antibodies. Besides, antibody levels against mosquito salivary proteins in malaria patients were highly variable, which may be related to the levels of mosquito exposure. Despite variability, patients' sera with high IgG titers consistently detected several proteins in Anopheles dirus salivary gland protein extracts. Immunohistochemical staining of Anopheles salivary glands with human sera showed that the salivary gland-specific IgGs reacted strongly with the median lobe. Comparison using Anopheles and Aedes salivary proteins suggests that the anti-salivary protein antibodies detected in malaria patients were Anopheles-specific, consistent with the major malaria vector status of An. dirus in this area.
AB - During blood feeding, arthropod vectors inject saliva into vertebrate hosts. The saliva is biochemically complex and pharmacologically active, and may play an important role in pathogen transmission. To examine whether mosquito saliva could elicit humoral immune response in humans under natural conditions, we have collected sera from malaria patients, healthy villagers, and people from a non-malarious region in Thailand. Here we have demonstrated that anti-Anopheles salivary protein antibodies occurred predominantly in patients with acute Plasmodium falciparum or P. vivax malaria, whereas people from a non-malarious area had no such antibodies. Besides, antibody levels against mosquito salivary proteins in malaria patients were highly variable, which may be related to the levels of mosquito exposure. Despite variability, patients' sera with high IgG titers consistently detected several proteins in Anopheles dirus salivary gland protein extracts. Immunohistochemical staining of Anopheles salivary glands with human sera showed that the salivary gland-specific IgGs reacted strongly with the median lobe. Comparison using Anopheles and Aedes salivary proteins suggests that the anti-salivary protein antibodies detected in malaria patients were Anopheles-specific, consistent with the major malaria vector status of An. dirus in this area.
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U2 - 10.1016/j.actatropica.2006.02.004
DO - 10.1016/j.actatropica.2006.02.004
M3 - Article
C2 - 16530153
AN - SCOPUS:33646853171
SN - 0001-706X
VL - 98
SP - 66
EP - 73
JO - Acta Tropica
JF - Acta Tropica
IS - 1
ER -
