Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription

Malini Natarajan; Gillian M. Schiralli Lester; Chanhyo Lee; Anamika Missra; Gregory A. Wasserman; Martin Steffen; David S. Gilmour; Andrew J. Henderson

doi:10.1074/jbc.M113.496489

Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription

Malini Natarajan
, Gillian M. Schiralli Lester
, Chanhyo Lee
, Anamika Missra
, Gregory A. Wasserman
, Martin Steffen
, David S. Gilmour
, Andrew J. Henderson

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Background: Multiple mechanisms contribute to HIV latency, including NELF-mediated RNA polymerase II (RNAP II) pausing. Results: Paused RNAP II recruits a transcription termination factor and a transcriptional corepressor complex to the HIV promoter. Conclusion: PausedRNAPII couples premature transcription termination and chromatin remodeling to maintain HIV latency. Significance: Paused RNAP II may be targeted to purge latent HIV infection.

Original language	English (US)
Pages (from-to)	25995-26003
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	36
DOIs	https://doi.org/10.1074/jbc.M113.496489
State	Published - Sep 6 2013

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M113.496489

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Natarajan, M., Schiralli Lester, G. M., Lee, C., Missra, A., Wasserman, G. A., Steffen, M., Gilmour, D. S., & Henderson, A. J. (2013). Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription. Journal of Biological Chemistry, 288(36), 25995-26003. https://doi.org/10.1074/jbc.M113.496489

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abstract = "Background: Multiple mechanisms contribute to HIV latency, including NELF-mediated RNA polymerase II (RNAP II) pausing. Results: Paused RNAP II recruits a transcription termination factor and a transcriptional corepressor complex to the HIV promoter. Conclusion: PausedRNAPII couples premature transcription termination and chromatin remodeling to maintain HIV latency. Significance: Paused RNAP II may be targeted to purge latent HIV infection.",

author = "Malini Natarajan and \{Schiralli Lester\}, \{Gillian M.\} and Chanhyo Lee and Anamika Missra and Wasserman, \{Gregory A.\} and Martin Steffen and Gilmour, \{David S.\} and Henderson, \{Andrew J.\}",

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doi = "10.1074/jbc.M113.496489",

language = "English (US)",

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Natarajan, M, Schiralli Lester, GM, Lee, C, Missra, A, Wasserman, GA, Steffen, M, Gilmour, DS & Henderson, AJ 2013, 'Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription', Journal of Biological Chemistry, vol. 288, no. 36, pp. 25995-26003. https://doi.org/10.1074/jbc.M113.496489

Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription. / Natarajan, Malini; Schiralli Lester, Gillian M.; Lee, Chanhyo et al.
In: Journal of Biological Chemistry, Vol. 288, No. 36, 06.09.2013, p. 25995-26003.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription

AU - Natarajan, Malini

AU - Schiralli Lester, Gillian M.

AU - Lee, Chanhyo

AU - Missra, Anamika

AU - Wasserman, Gregory A.

AU - Steffen, Martin

AU - Gilmour, David S.

AU - Henderson, Andrew J.

PY - 2013/9/6

Y1 - 2013/9/6

N2 - Background: Multiple mechanisms contribute to HIV latency, including NELF-mediated RNA polymerase II (RNAP II) pausing. Results: Paused RNAP II recruits a transcription termination factor and a transcriptional corepressor complex to the HIV promoter. Conclusion: PausedRNAPII couples premature transcription termination and chromatin remodeling to maintain HIV latency. Significance: Paused RNAP II may be targeted to purge latent HIV infection.

AB - Background: Multiple mechanisms contribute to HIV latency, including NELF-mediated RNA polymerase II (RNAP II) pausing. Results: Paused RNAP II recruits a transcription termination factor and a transcriptional corepressor complex to the HIV promoter. Conclusion: PausedRNAPII couples premature transcription termination and chromatin remodeling to maintain HIV latency. Significance: Paused RNAP II may be targeted to purge latent HIV infection.

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 36

ER -

Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription

Abstract

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