Nested Biofabrication: Matryoshka-Inspired Intra-Embedded Bioprinting

Mecit Altan Alioglu, Yasar Ozer Yilmaz, Yogendra Pratap Singh, Momoka Nagamine, Nazmiye Celik, Myoung Hwan Kim, Vaibhav Pal, Deepak Gupta, Ibrahim T. Ozbolat

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Engineering functional tissues and organs remains a fundamental pursuit in bio-fabrication. However, the accurate constitution of complex shapes and internal anatomical features of specific organs, including their intricate blood vessels and nerves, remains a significant challenge. Inspired by the Matryoshka doll, here a new method called “Intra-Embedded Bioprinting (IEB)” is introduced building upon existing embedded bioprinting methods. a xanthan gum-based material is used which served a dual role as both a bioprintable ink and a support bath, due to its unique shear-thinning and self-healing properties. IEB's capabilities in organ modeling, creating a miniaturized replica of a pancreas using a photocrosslinkable silicone composite is demonstrated. Further, a head phantom and a Matryoshka doll are 3D printed, exemplifying IEB's capability to manufacture intricate, nested structures. Toward the use case of IEB and employing an innovative coupling strategy between extrusion-based and aspiration-assisted bioprinting, a breast tumor model that included a central channel mimicking a blood vessel, with tumor spheroids bioprinted in proximity is developed. Validation using a clinically-available chemotherapeutic drug illustrated its efficacy in reducing the tumor volume via perfusion over time. This method opens a new way of bioprinting enabling the creation of complex-shaped organs with internal anatomical features.

Original languageEnglish (US)
JournalSmall Methods
StateAccepted/In press - 2023

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Materials Science

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