Neurogenic differentiation of murine and human adipose-derived stromal cells

Kristine M. Safford; Kevin C. Hicok; Shawn D. Safford; Yuan Di C. Halvorsen; William O. Wilkison; Jeffrey M. Gimble; Henry E. Rice

doi:10.1016/S0006-291X(02)00469-2

Neurogenic differentiation of murine and human adipose-derived stromal cells

Kristine M. Safford, Kevin C. Hicok, Shawn D. Safford, Yuan Di C. Halvorsen, William O. Wilkison, Jeffrey M. Gimble, Henry E. Rice

Department of Surgery

Research output: Contribution to journal › Article › peer-review

712 Scopus citations

Abstract

The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid butylated hydroxyanisole insulin and hydrocortisone. As early as 1-3 h after neuronal induction the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction muADAS cells displayed immunocytochemical staining for GFAP nestin and NeuN and huADAS cells displayed staining for intermediate filament M nestin and NeuN. Following neuronal induction of murine and human ADAS cells Western blot analysis confirmed GFAP nestin and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.

Original language	English (US)
Pages (from-to)	371-379
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	294
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(02)00469-2
State	Published - 2002

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Neurogenic differentiation of murine and human adipose-derived stromal cells",

abstract = "The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid butylated hydroxyanisole insulin and hydrocortisone. As early as 1-3 h after neuronal induction the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction muADAS cells displayed immunocytochemical staining for GFAP nestin and NeuN and huADAS cells displayed staining for intermediate filament M nestin and NeuN. Following neuronal induction of murine and human ADAS cells Western blot analysis confirmed GFAP nestin and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.",

author = "Safford, {Kristine M.} and Hicok, {Kevin C.} and Safford, {Shawn D.} and Halvorsen, {Yuan Di C.} and Wilkison, {William O.} and Gimble, {Jeffrey M.} and Rice, {Henry E.}",

note = "Funding Information: This work was supported by funds provided by the Owen H. Wagensteen, MD Faculty Research Fellowship of the American College of Surgeons, as well as by funds provided by Artecel Sciences, Inc. Dr. Rice serves as a consultant to Artecel Sciences, Inc. Note that portions of this research were presented at the 33rd Annual Meeting of the American Pediatric Surgical Association, May, 2002. The authors thank Alex Freemerman and Ashok Shetty for expert advice during the course of this work. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "2002",

doi = "10.1016/S0006-291X(02)00469-2",

language = "English (US)",

volume = "294",

pages = "371--379",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

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T1 - Neurogenic differentiation of murine and human adipose-derived stromal cells

AU - Safford, Kristine M.

AU - Hicok, Kevin C.

AU - Safford, Shawn D.

AU - Halvorsen, Yuan Di C.

AU - Wilkison, William O.

AU - Gimble, Jeffrey M.

AU - Rice, Henry E.

N1 - Funding Information: This work was supported by funds provided by the Owen H. Wagensteen, MD Faculty Research Fellowship of the American College of Surgeons, as well as by funds provided by Artecel Sciences, Inc. Dr. Rice serves as a consultant to Artecel Sciences, Inc. Note that portions of this research were presented at the 33rd Annual Meeting of the American Pediatric Surgical Association, May, 2002. The authors thank Alex Freemerman and Ashok Shetty for expert advice during the course of this work. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid butylated hydroxyanisole insulin and hydrocortisone. As early as 1-3 h after neuronal induction the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction muADAS cells displayed immunocytochemical staining for GFAP nestin and NeuN and huADAS cells displayed staining for intermediate filament M nestin and NeuN. Following neuronal induction of murine and human ADAS cells Western blot analysis confirmed GFAP nestin and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.

AB - The identification of cells capable of neuronal differentiation has great potential for cellular therapies. We examined whether murine and human adipose-derived adult stem (ADAS) cells can be induced to undergo neuronal differentiation. We isolated ADAS cells from the adipose tissue of adult BalbC mice or from human liposuction tissue and induced neuronal differentiation with valproic acid butylated hydroxyanisole insulin and hydrocortisone. As early as 1-3 h after neuronal induction the phenotype of ADAS cells changed towards neuronal morphology. Following neuronal induction muADAS cells displayed immunocytochemical staining for GFAP nestin and NeuN and huADAS cells displayed staining for intermediate filament M nestin and NeuN. Following neuronal induction of murine and human ADAS cells Western blot analysis confirmed GFAP nestin and NeuN protein expression. Pretreatment with EGF and basic FGF augmented the neuronal differentiation of huADAS cells. The neuronal differentiation of stromal cells from adipose tissue has broad biological and clinical implications.

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Neurogenic differentiation of murine and human adipose-derived stromal cells

Abstract

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