TY - JOUR
T1 - Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon
AU - Hirai, Keiji
AU - Browning, Kirsteen N.
AU - Lees, Gordon M.
N1 - Funding Information:
The financial assistance of the Welcome Trust, and a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan is gratefully acknowledged.
PY - 1997/5/23
Y1 - 1997/5/23
N2 - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.
AB - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.
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U2 - 10.1016/S0304-3940(97)00325-X
DO - 10.1016/S0304-3940(97)00325-X
M3 - Article
C2 - 9185688
AN - SCOPUS:0030907083
SN - 0304-3940
VL - 227
SP - 212
EP - 214
JO - Neuroscience letters
JF - Neuroscience letters
IS - 3
ER -