Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

Keiji Hirai; Kirsteen N. Browning; Gordon M. Lees

doi:10.1016/S0304-3940(97)00325-X

Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

Keiji Hirai
, Kirsteen N. Browning
, Gordon M. Lees

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α₂-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro³⁴-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y₁-receptor and increasing potassium conductance.

Original language	English (US)
Pages (from-to)	212-214
Number of pages	3
Journal	Neuroscience letters
Volume	227
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(97)00325-X
State	Published - May 23 1997

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/S0304-3940(97)00325-X

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@article{ce8a121e8a5943c0853a2348f93c32f0,

title = "Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon",

abstract = "Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90\%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.",

author = "Keiji Hirai and Browning, \{Kirsteen N.\} and Lees, \{Gordon M.\}",

note = "Funding Information: The financial assistance of the Welcome Trust, and a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan is gratefully acknowledged.",

year = "1997",

month = may,

day = "23",

doi = "10.1016/S0304-3940(97)00325-X",

language = "English (US)",

volume = "227",

pages = "212--214",

journal = "Neuroscience letters",

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publisher = "Elsevier Ireland Ltd",

number = "3",

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TY - JOUR

T1 - Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

AU - Hirai, Keiji

AU - Browning, Kirsteen N.

AU - Lees, Gordon M.

N1 - Funding Information: The financial assistance of the Welcome Trust, and a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan is gratefully acknowledged.

PY - 1997/5/23

Y1 - 1997/5/23

N2 - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.

AB - Effects of neuropeptide Y (NPY) on submucosal neurons of the guinea-pig descending colon were investigated electrophysiologically by means of intracellular electrophysiological recordings. NPY (100 nM) induced a marked and prolonged hyperpolarization, accompanied by a decrease in input resistance in most (90%) neurons. This NPY-induced hyperpolarization was diminished and augmented by membrane hyperpolarization and depolarization, respectively. The NPY-hyperpolarization was not affected by exposure to either calcium-free solu solutions or the α2-adrenoceptor antagonist, idazoxan (1 μM). When more than one peptide was applied to a neuron, NPY, PYY and Pro34-NPY were equipotent, whilst NPY13-36 was less potent. It was concluded that NPY hyperpolarized submucosal neurons of the guinea-pig descending colon, possibly via a direct action on postsynaptic Y1-receptor and increasing potassium conductance.

UR - https://www.scopus.com/pages/publications/0030907083

UR - https://www.scopus.com/pages/publications/0030907083#tab=citedBy

U2 - 10.1016/S0304-3940(97)00325-X

DO - 10.1016/S0304-3940(97)00325-X

M3 - Article

C2 - 9185688

AN - SCOPUS:0030907083

SN - 0304-3940

VL - 227

SP - 212

EP - 214

JO - Neuroscience letters

JF - Neuroscience letters

IS - 3

ER -

Neuropeptide Y hyperpolarizes submucosal neurons of the guinea-pig descending colon

Abstract

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