New rapid method to detect BCR-ABL fusion genes with multiplex RT-qPCR in one-tube at a time

Yong Qing Tong, Zhi Jun Zhao, Bei Liu, An Yu Bao, Hong Yun Zheng, Jian Gu, Ying Xia, Mary McGrath, Sinisa Dovat, Chunhua Song, Yan Li

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Fast identification of BCR-ABL fusion genes is critical for precise diagnosis, risk stratification and therapy scheme selection in leukemia. More convenient methods are needed for quickly detection of the BCR-ABL fusion genes. Multiplex fluorescent reverse transcription quantitative real-time PCR (Multiplex RT-qPCR) methods are developed for detection of the at least 14 subtypes of BCR-ABL fusion genes in one tube at a time by using patients’ bone marrow samples. The new Multiplex RT-qPCR method could quickly detect BCR-ABL fusion genes with sensitivity up to 10–10 6 copies. It can detect the fusion genes in patients’ bone marrow samples containing any subtypes of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (μ-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The specificity is comparable to the FISH methods. The cutoff for clinical diagnosis of BCR-ABL(+) is also determined by testing in clinical chronic myeloid leukemia samples. This is a new fast method with high sensitivity and specificity for clinical detection of BCR-ABL fusion genes. It will benefit the precise diagnosis, targeted therapy and minimal residual disease (MRD) monitoring in leukemia.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalLeukemia Research
StatePublished - Jun 2018

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research


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