TY - JOUR
T1 - Next-generation sequencing (Ngs) in covid-19
T2 - A tool for sars-cov-2 diagnosis, monitoring new strains and phylodynamic modeling in molecular epidemiology
AU - John, Goldin
AU - Sahajpal, Nikhil Shri
AU - Mondal, Ashis K.
AU - Ananth, Sudha
AU - Williams, Colin
AU - Chaubey, Alka
AU - Rojiani, Amyn M.
AU - Kolhe, Ravindra
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9
Y1 - 2021/9
N2 - This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
AB - This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
UR - http://www.scopus.com/inward/record.url?scp=85111783394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85111783394&partnerID=8YFLogxK
U2 - 10.3390/cimb43020061
DO - 10.3390/cimb43020061
M3 - Review article
C2 - 34449545
AN - SCOPUS:85111783394
SN - 1467-3037
VL - 43
SP - 845
EP - 867
JO - Current Issues in Molecular Biology
JF - Current Issues in Molecular Biology
IS - 2
ER -