NMR analysis reveals a wealth of metabolites in Root-Knot nematode resistant roots of Citrullus amarus watermelon plants

Mihail Kantor, Amnon Levi, Judith Thies, Nihat Guner, Camelia Kantor, Stuart Parnham, Arezue Boroujerdi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Citrullus amarus (CA) (previously known as Citrullus lanatus var. citroides) accessions collected in southern Africa are known to have resistance to root-knot nematodes (RKN) and are suitable rootstocks for grafted watermelon. The objective of this study was to conduct a comparative metabolomics analysis and identify unique metabolites in roots of CA accessions versus roots of watermelon cultivars (Citrullus lanatus (Thunb.) Matsum. and Nakai var. lanatus; CL). Nuclear magnetic resonance (NMR) technology and principal component analysis (PCA) were used to analyze and compare metabolic profiles of seven CA accessions resistant to RKN along with two RKNsusceptible watermelon cultivars (Charleston Gray and Crimson Sweet). Calculation of the Mahalanobis distance revealed that the CA United States Plant Introduction (PI) 189225 (Line number 1832) and PI 482324 (1849) have the most distinct metabolic profiles compared with the watermelon cultivars Charleston Gray and Crimson Sweet, respectively. Several amino acids identified in the CA accessions were reported in previous studies to have a nematicidal effect. The results in this study indicate that roots of watermelon accessions collected in the wild are rich in metabolic compounds. These metabolic compounds may have been diminished in watermelon cultivars as a consequence of many years of cultivation and selection for desirable fruit qualities.

Original languageEnglish (US)
Pages (from-to)303-316
Number of pages14
JournalJournal of Nematology
Volume50
Issue number3
DOIs
StatePublished - 2018

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

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