TY - JOUR
T1 - NOD2 mutations affect muramyl dipeptide stimulation of human B lymphocytes and interact with other IBD-associated genes
AU - Lin, Zhenwu
AU - Hegarty, John P.
AU - John, Gerrit
AU - Berg, Arthur
AU - Wang, Zhong
AU - Sehgal, Rishabh
AU - Pastor, Danielle M.
AU - Wang, Yunhua
AU - Harris, Leonard R.
AU - Poritz, Lisa S.
AU - Schreiber, Stefan
AU - Koltun, Walter A.
N1 - Funding Information:
Acknowledgments The authors thank Tony Lin for his assistant with RFLP genotyping. This work is supported by a grant from the Philadelphia Health Care Trust (to W.A Koltun) and a Surgery Initiation Grant from Pennsylvania State University College of Medicine (to Z. Lin).
PY - 2013/9
Y1 - 2013/9
N2 - Background: Genetic and functional studies have associated variants in the NOD2/CARD15 gene with Crohn's disease. Aims: This study aims to replicate the association of three common NOD2 mutations with Crohn's disease, study its effect on NOD2 expression in B cells and its interaction with other IBD-associated genes. Methods: A total of 294 IBD patients (179 familial IBD, 115 sporadic IBD) and 298 unrelated healthy controls were from central Pennsylvania. NOD2 mutations were analyzed by primer-specific amplification, PCR based-RFLP, and validated with the ABI SNPlexM genotyping system. Gene-gene interaction was studied using a statistical model for epistasis analysis. Results: Three common NOD2 mutations are associated with Crohn's disease (p = 5.08 × 10-7, 1.67 × 10-6, and 1.87 × 10-2 for 1007fs, R720W, and G908R, respectively), but not with ulcerative colitis (p = 0.1046, 0.1269, and 0.8929, respectively). For IBD overall, 1007finsC (p = 4.4 × 10-5) and R720W (p = 9.24 × 10-5) were associated with IBD, but not G908R (p = 0.1198). We revealed significant interactions of NOD2 with other IBD susceptibility genes IL23R, DLG5, and OCTN1. We discovered that NOD2 was expressed in both normal human peripheral blood B cells and in EBV-transformed B cell lines. Moreover, we further demonstrated that muramyl dipeptide (MDP) stimulation of B lymphocytes up-regulated expression of NF-κB-p50 mRNA. Conclusion: NOD2 is expressed in peripheral B cells, and the up-regulation of NOD2 expression by MDP was significantly impaired by NOD2 mutations. The finding suggests a possible role of NOD2 in the immunological response in IBD pathogenesis.
AB - Background: Genetic and functional studies have associated variants in the NOD2/CARD15 gene with Crohn's disease. Aims: This study aims to replicate the association of three common NOD2 mutations with Crohn's disease, study its effect on NOD2 expression in B cells and its interaction with other IBD-associated genes. Methods: A total of 294 IBD patients (179 familial IBD, 115 sporadic IBD) and 298 unrelated healthy controls were from central Pennsylvania. NOD2 mutations were analyzed by primer-specific amplification, PCR based-RFLP, and validated with the ABI SNPlexM genotyping system. Gene-gene interaction was studied using a statistical model for epistasis analysis. Results: Three common NOD2 mutations are associated with Crohn's disease (p = 5.08 × 10-7, 1.67 × 10-6, and 1.87 × 10-2 for 1007fs, R720W, and G908R, respectively), but not with ulcerative colitis (p = 0.1046, 0.1269, and 0.8929, respectively). For IBD overall, 1007finsC (p = 4.4 × 10-5) and R720W (p = 9.24 × 10-5) were associated with IBD, but not G908R (p = 0.1198). We revealed significant interactions of NOD2 with other IBD susceptibility genes IL23R, DLG5, and OCTN1. We discovered that NOD2 was expressed in both normal human peripheral blood B cells and in EBV-transformed B cell lines. Moreover, we further demonstrated that muramyl dipeptide (MDP) stimulation of B lymphocytes up-regulated expression of NF-κB-p50 mRNA. Conclusion: NOD2 is expressed in peripheral B cells, and the up-regulation of NOD2 expression by MDP was significantly impaired by NOD2 mutations. The finding suggests a possible role of NOD2 in the immunological response in IBD pathogenesis.
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U2 - 10.1007/s10620-013-2696-8
DO - 10.1007/s10620-013-2696-8
M3 - Article
C2 - 23709157
AN - SCOPUS:84884209193
SN - 0163-2116
VL - 58
SP - 2599
EP - 2607
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 9
ER -
