Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc

Gihoon Choi; Weihua Guan

doi:10.1109/TRANSDUCERS.2019.8808740

Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc

Gihoon Choi, Weihua Guan

Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Most lab-on-a-disc platforms use centrifugal force to pump liquids. In this work, we demonstrated a novel energy efficient non-centrifugal lab-on-a-disc nucleic acid testing device in which no liquid motion is involved. Streamlined DNA extraction, amplification, and real-time detection on a single microfluidic reagent disc are achieved by actuating the DNA-carrying magnetic beads against stationary reagent droplets. Using malaria spiked whole blood as a testing model, we demonstrated an excellent detection limit of 0.5 parasites/μl, sufficient for detecting asymptomatic malaria parasite carriers.

Original language	English (US)
Title of host publication	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	118-121
Number of pages	4
ISBN (Electronic)	9781728120072
DOIs	https://doi.org/10.1109/TRANSDUCERS.2019.8808740
State	Published - Jun 1 2019
Event	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany Duration: Jun 23 2019 → Jun 27 2019

Publication series

Name	2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference	20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
Country/Territory	Germany
City	Berlin
Period	6/23/19 → 6/27/19

All Science Journal Classification (ASJC) codes

Process Chemistry and Technology
Spectroscopy
Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Control and Optimization
Instrumentation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/TRANSDUCERS.2019.8808740

Cite this

Choi, G., & Guan, W. (2019). Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 118-121). Article 8808740 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808740

Choi, Gihoon ; Guan, Weihua. / Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 118-121 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

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abstract = "Most lab-on-a-disc platforms use centrifugal force to pump liquids. In this work, we demonstrated a novel energy efficient non-centrifugal lab-on-a-disc nucleic acid testing device in which no liquid motion is involved. Streamlined DNA extraction, amplification, and real-time detection on a single microfluidic reagent disc are achieved by actuating the DNA-carrying magnetic beads against stationary reagent droplets. Using malaria spiked whole blood as a testing model, we demonstrated an excellent detection limit of 0.5 parasites/μl, sufficient for detecting asymptomatic malaria parasite carriers.",

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booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",

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Choi, G & Guan, W 2019, Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808740, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 118-121, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 6/23/19. https://doi.org/10.1109/TRANSDUCERS.2019.8808740

Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc. / Choi, Gihoon; Guan, Weihua.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 118-121 8808740 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - Most lab-on-a-disc platforms use centrifugal force to pump liquids. In this work, we demonstrated a novel energy efficient non-centrifugal lab-on-a-disc nucleic acid testing device in which no liquid motion is involved. Streamlined DNA extraction, amplification, and real-time detection on a single microfluidic reagent disc are achieved by actuating the DNA-carrying magnetic beads against stationary reagent droplets. Using malaria spiked whole blood as a testing model, we demonstrated an excellent detection limit of 0.5 parasites/μl, sufficient for detecting asymptomatic malaria parasite carriers.

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Choi G, Guan W. Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 118-121. 8808740. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). doi: 10.1109/TRANSDUCERS.2019.8808740

Non-Centrifugal Microfluidic Nucleic Acid Testing on Lab-on-a-Disc

Abstract

Publication series

Conference

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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