Novel anti-factor D antibody inhibits complement, neutrophil, and platelet activation in a simulated pediatric cardiopulmonary bypass circuit

A study using human blood in a simulated pediatric cardiopulmonary bypass (CPB) circuit was carried out to investigate the effects of an anti-factor D monoclonal antibody on inhibition of the alternative complement cascade. Five paired experiments were performed on Mab 166-32 and an irrelevant control Mab at 18 μg/ml of human blood. Activation of complement, neutrophils, and platelets was assessed with immunofluorocytometry and ELISA. Complement activation via the alternative complement pathway induced the inflammatory responses in CPB, which were effectively inhibited by the anti-factor D Mab 166-32.