TY - JOUR
T1 - Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7 Å resolution
AU - Gaiser, Florian
AU - Tan, Song
AU - Richmond, Timothy J.
N1 - Funding Information:
We thank D. Sargent for assistance in data collection and the staff at beamlines ID14-4 and BM14 of the ESRF, Grenoble for excellent technical support. R. Coleman and I. Berger are acknowledged for helpful discussion on the manuscript. This work was supported in part by the Swiss National Science Foundation and the Novartis Stiftung.
PY - 2000/10/6
Y1 - 2000/10/6
N2 - General transcription factor IIF (TFIIF) is required for transcription by RNA polymerase II; it consists minimally of a heterodimer of RNA polymerase-associated proteins RAP30 and RAP74. According to solution and mutagenesis studies, the multiple domains of RAP30 and RAP74 bind PolII, TFIIB, TAF250 and DNA in interactions that are essential for transcription initiation and elongation. The X-ray structure of the RAP30/RAP74 interaction domains at 1.7 Å resolution reveals a novel 'triple barrel' dimerization fold and suggests with mutant data that interactions with the transcription apparatus are mediated not only by this tripartite β-barrel, but also via flexible loops and α and β-structures extending from it. (C) 2000 Academic Press.
AB - General transcription factor IIF (TFIIF) is required for transcription by RNA polymerase II; it consists minimally of a heterodimer of RNA polymerase-associated proteins RAP30 and RAP74. According to solution and mutagenesis studies, the multiple domains of RAP30 and RAP74 bind PolII, TFIIB, TAF250 and DNA in interactions that are essential for transcription initiation and elongation. The X-ray structure of the RAP30/RAP74 interaction domains at 1.7 Å resolution reveals a novel 'triple barrel' dimerization fold and suggests with mutant data that interactions with the transcription apparatus are mediated not only by this tripartite β-barrel, but also via flexible loops and α and β-structures extending from it. (C) 2000 Academic Press.
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U2 - 10.1006/jmbi.2000.4110
DO - 10.1006/jmbi.2000.4110
M3 - Article
C2 - 11183778
AN - SCOPUS:0034613012
SN - 0022-2836
VL - 302
SP - 1119
EP - 1127
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 5
ER -