Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes

Michael Bleichenbacher, Song Tan, Timothy J. Richmond

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1Å and 1.9Å resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.

Original languageEnglish (US)
Pages (from-to)783-793
Number of pages11
JournalJournal of Molecular Biology
Issue number4
StatePublished - Sep 26 2003

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology


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