TY - JOUR
T1 - Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes
AU - Bleichenbacher, Michael
AU - Tan, Song
AU - Richmond, Timothy J.
N1 - Funding Information:
We thank Peter Hackel and Arne Woern for their important contributions at an early stage in this project. We are grateful to the staff of the EMBL Hamburg Outstation at DESY for their assistance during data collection. We appreciate the support of the Swiss National Fund.
PY - 2003/9/26
Y1 - 2003/9/26
N2 - RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1Å and 1.9Å resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.
AB - RNA polymerase II-dependent transcription requires the assembly of a multi-protein, preinitiation complex on core promoter elements. Transcription factor IID (TFIID) comprising the TATA box-binding protein (TBP) and TBP-associated factors (TAFs) is responsible for promoter recognition in this complex. Subsequent association of TFIIA and TFIIB provides enhanced complex stability. TFIIA is required for transcriptional stimulation by certain viral and cellular activators, and favors formation of the preinitiation complex in the presence of repressor NC2. The X-ray structures of human and yeast TBP/TFIIA/DNA complexes at 2.1Å and 1.9Å resolution, respectively, are presented here and seen to resemble each other closely. The interactions made by human TFIIA with TBP and DNA within and upstream of the TATA box, including those involving water molecules, are described and compared to the yeast structure. Of particular interest is a previously unobserved region of TFIIA that extends the binding interface with TBP in the yeast, but not in the human complex, and that further elucidates biochemical and genetic results.
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U2 - 10.1016/S0022-2836(03)00887-8
DO - 10.1016/S0022-2836(03)00887-8
M3 - Article
C2 - 12972251
AN - SCOPUS:0041825432
SN - 0022-2836
VL - 332
SP - 783
EP - 793
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -