TY - JOUR
T1 - Novel splice variant of mouse insulin2 mRNA
T2 - Implications for insulin expression
AU - Panda, Amaresh C.
AU - Kulkarni, Shardul D.
AU - Muralidharan, Bhavana
AU - Bakthavachalu, Baskar
AU - Seshadri, Vasudevan
N1 - Funding Information:
This work was supported by grant from DBT, India ( BT/PR5813/BRB/10/411/2005 ), and intramural grant from NCCS, India. S.D.K., B.M., and A.C.P. are supported by a fellowship from CSIR, India, and B.B. is supported by a fellowship from ICMR, India.
PY - 2010/3
Y1 - 2010/3
N2 - Insulin is a secreted peptide that controls glucose homeostasis in mammals, and insulin biosynthesis is regulated by glucose at many levels. Rodent insulin is encoded by two non-allelic genes. We have identified a novel splice variant of the insulin2 gene in mice that constitutes about 75% of total insulin2 mRNA. The alternate splicing does not alter the ORF but reduces the 5′UTR by 12 bases. A reporter gene containing the novel short 5′UTR, is more efficiently expressed in cells, suggesting that alternative splicing of insulin mRNA in mice could result in an additional level of regulation in insulin biosynthesis.
AB - Insulin is a secreted peptide that controls glucose homeostasis in mammals, and insulin biosynthesis is regulated by glucose at many levels. Rodent insulin is encoded by two non-allelic genes. We have identified a novel splice variant of the insulin2 gene in mice that constitutes about 75% of total insulin2 mRNA. The alternate splicing does not alter the ORF but reduces the 5′UTR by 12 bases. A reporter gene containing the novel short 5′UTR, is more efficiently expressed in cells, suggesting that alternative splicing of insulin mRNA in mice could result in an additional level of regulation in insulin biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=77950369505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950369505&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2010.02.020
DO - 10.1016/j.febslet.2010.02.020
M3 - Article
C2 - 20153322
AN - SCOPUS:77950369505
SN - 0014-5793
VL - 584
SP - 1169
EP - 1173
JO - FEBS Letters
JF - FEBS Letters
IS - 6
ER -