Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica

Shannon E. Julian; Tim L. King

doi:10.1046/j.1471-8286.2003.00417.x

Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica

Shannon E. Julian, Tim L. King

Ecosystem Science and Management

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24 Scopus citations

Abstract

Fifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6-92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all F_ST values (0.02-0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and metapopulation dynamics.

Original language	English (US)
Pages (from-to)	256-258
Number of pages	3
Journal	Molecular Ecology Notes
Volume	3
Issue number	2
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00417.x
State	Published - Jun 2003

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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10.1046/j.1471-8286.2003.00417.x

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abstract = "Fifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6-92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all FST values (0.02-0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and metapopulation dynamics.",

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AB - Fifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6-92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all FST values (0.02-0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and metapopulation dynamics.

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Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica

Abstract

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