Novel tetranucleotide microsatellite DNA markers for the wood frog, Rana sylvatica

Shannon E. Julian, Tim L. King

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Fifteen tetranucleotide microsatellite loci were identified and characterized for wood frogs (Rana sylvatica) collected from three vernal pools in the southeastern US. These markers revealed a high degree of genetic diversity (nine to 34 alleles per locus), heterozygosity (30.6-92.3%) and allelic heterogeneity (69% of comparisons were statistically significant). Considerable differentiation among populations was observed as genetic distances (chord) ranged between 0.40 and 0.55 and all FST values (0.02-0.05) were statistically significant. Genotypic assignment tests correctly classified 103 of 113 individuals to their respective collection. These markers should prove useful for investigating fine-scale population structure and metapopulation dynamics.

Original languageEnglish (US)
Pages (from-to)256-258
Number of pages3
JournalMolecular Ecology Notes
Issue number2
StatePublished - Jun 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • General Biochemistry, Genetics and Molecular Biology


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