TY - JOUR
T1 - NPK1, and MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants
AU - Jin, Hailing
AU - Axtell, Michael J.
AU - Dahlbeck, Douglas
AU - Ekwenna, Obi
AU - Zhang, Shuqun
AU - Staskawicz, Brian
AU - Baker, Barbara
N1 - Funding Information:
We thank Jennifer Fletcher for valuable advice and critical reading of the manuscript, David Baulcombe for the PVX and the TRV vectors, Jen Sheen for the constitutively active NPK1 plasmids and helpful discussions, Pierre De Wit for pCf4 and pAvrCf4 constructs, and Vanessa Handley and Miki Yamamoto for critical reading of the manuscript. This work was supported by USDA CRIS 5335-22000-005-00D and USDA-ARS/Syngenta CRADA 58-3K95-0-829.
PY - 2002/8
Y1 - 2002/8
N2 - Mitogen-activated protein kinase (MAPK) cascades are rapidly activated upon plant recognition of invading pathogens. Here, we describe the use of virus-induced gene silencing (VIGS) to study the role of candidate plant MAP kinase kinase kinase (MAPKKK) homologs of human MEKK1 in pathogen-resistance pathways. We demonstrate that silencing expression of a tobacco MAPKKK, Nicotiana Protein Kinase 1 (NPK1), interferes with the function of the disease-resistance genes N, Bs2, and Rx, but does not affect Pto- and Cf4-mediated resistance. Further, NPK1-silenced plants also exhibit reduced cell size, defective cytokinesis, and an overall dwarf phenotype. Our results provide evidence that NPK1 functions in the regulation of N-, Bs2-, and Rx-mediated resistance responses and may play a role in one or more MAPK cascades, regulating multiple cellular processes.
AB - Mitogen-activated protein kinase (MAPK) cascades are rapidly activated upon plant recognition of invading pathogens. Here, we describe the use of virus-induced gene silencing (VIGS) to study the role of candidate plant MAP kinase kinase kinase (MAPKKK) homologs of human MEKK1 in pathogen-resistance pathways. We demonstrate that silencing expression of a tobacco MAPKKK, Nicotiana Protein Kinase 1 (NPK1), interferes with the function of the disease-resistance genes N, Bs2, and Rx, but does not affect Pto- and Cf4-mediated resistance. Further, NPK1-silenced plants also exhibit reduced cell size, defective cytokinesis, and an overall dwarf phenotype. Our results provide evidence that NPK1 functions in the regulation of N-, Bs2-, and Rx-mediated resistance responses and may play a role in one or more MAPK cascades, regulating multiple cellular processes.
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U2 - 10.1016/S1534-5807(02)00205-8
DO - 10.1016/S1534-5807(02)00205-8
M3 - Article
C2 - 12194859
AN - SCOPUS:0036696447
SN - 1534-5807
VL - 3
SP - 291
EP - 297
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -