Nuclear magnetic resonance study of free and bound water fractions in normal lenses

P. J. Stankeiwicz; K. R. Metz; J. W. Sassani; R. W. Briggs

Nuclear magnetic resonance study of free and bound water fractions in normal lenses

P. J. Stankeiwicz, K. R. Metz, J. W. Sassani, R. W. Briggs

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Abstract

Proton NMR relaxation times T₁ and T₂ were determined for normal lenses excised from sexually mature animals from seven different species. Lenses were immersed in physiological buffer during measurements, and suppression methods were employed to null the buffer signal. This enabled selective analysis of lenticular water. Observed relaxation times were correlated with protein and water content. At 37°C and 1.89 Tesla, single-exponential spin-lattice relaxation was observed, but spin-spin relaxation was found to be double-exponential. It was shown that the short-T₂ fraction is proportional to protein concentration; this fraction was attributed to water bound to protein. The long-T₂ fraction was attributed to free lenticular water. The amounts of free and bound water thus obtained were used in the spin-lattice relaxation rate equation for rapid exchange in a two-component system to calculate the magnitude of the two corresponding T₁ relaxation components.

Original language	English (US)
Pages (from-to)	2361-2369
Number of pages	9
Journal	Investigative Ophthalmology and Visual Science
Volume	30
Issue number	11
State	Published - 1989

All Science Journal Classification (ASJC) codes

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

Cite this

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title = "Nuclear magnetic resonance study of free and bound water fractions in normal lenses",

abstract = "Proton NMR relaxation times T1 and T2 were determined for normal lenses excised from sexually mature animals from seven different species. Lenses were immersed in physiological buffer during measurements, and suppression methods were employed to null the buffer signal. This enabled selective analysis of lenticular water. Observed relaxation times were correlated with protein and water content. At 37°C and 1.89 Tesla, single-exponential spin-lattice relaxation was observed, but spin-spin relaxation was found to be double-exponential. It was shown that the short-T2 fraction is proportional to protein concentration; this fraction was attributed to water bound to protein. The long-T2 fraction was attributed to free lenticular water. The amounts of free and bound water thus obtained were used in the spin-lattice relaxation rate equation for rapid exchange in a two-component system to calculate the magnitude of the two corresponding T1 relaxation components.",

author = "Stankeiwicz, {P. J.} and Metz, {K. R.} and Sassani, {J. W.} and Briggs, {R. W.}",

year = "1989",

language = "English (US)",

volume = "30",

pages = "2361--2369",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "11",

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TY - JOUR

T1 - Nuclear magnetic resonance study of free and bound water fractions in normal lenses

AU - Stankeiwicz, P. J.

AU - Metz, K. R.

AU - Sassani, J. W.

AU - Briggs, R. W.

PY - 1989

Y1 - 1989

N2 - Proton NMR relaxation times T1 and T2 were determined for normal lenses excised from sexually mature animals from seven different species. Lenses were immersed in physiological buffer during measurements, and suppression methods were employed to null the buffer signal. This enabled selective analysis of lenticular water. Observed relaxation times were correlated with protein and water content. At 37°C and 1.89 Tesla, single-exponential spin-lattice relaxation was observed, but spin-spin relaxation was found to be double-exponential. It was shown that the short-T2 fraction is proportional to protein concentration; this fraction was attributed to water bound to protein. The long-T2 fraction was attributed to free lenticular water. The amounts of free and bound water thus obtained were used in the spin-lattice relaxation rate equation for rapid exchange in a two-component system to calculate the magnitude of the two corresponding T1 relaxation components.

AB - Proton NMR relaxation times T1 and T2 were determined for normal lenses excised from sexually mature animals from seven different species. Lenses were immersed in physiological buffer during measurements, and suppression methods were employed to null the buffer signal. This enabled selective analysis of lenticular water. Observed relaxation times were correlated with protein and water content. At 37°C and 1.89 Tesla, single-exponential spin-lattice relaxation was observed, but spin-spin relaxation was found to be double-exponential. It was shown that the short-T2 fraction is proportional to protein concentration; this fraction was attributed to water bound to protein. The long-T2 fraction was attributed to free lenticular water. The amounts of free and bound water thus obtained were used in the spin-lattice relaxation rate equation for rapid exchange in a two-component system to calculate the magnitude of the two corresponding T1 relaxation components.

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JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 11

ER -

Nuclear magnetic resonance study of free and bound water fractions in normal lenses

Abstract

All Science Journal Classification (ASJC) codes

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