Nuclear Stiffening Inhibits Migration of Invasive Melanoma Cells

Alexandre J.S. Ribeiro, Payal Khanna, Aishwarya Sukumar, Cheng Dong, Kris Noel Dahl

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of Δ50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Melanoma cells, both WM35 and Lu1205, both show reduced nuclear deformability and reduced cell invasion with the expression of Δ50 lamin A. These studies suggest that cellular aging including expression of Δ50 lamin A and nuclear stiffening may reduce the potential for metastatic cancer migration. Thus, the pathway of cancer metastasis may be kept in check by mechanical factors in addition to known chemical pathway regulation.

Original languageEnglish (US)
Pages (from-to)544-551
Number of pages8
JournalCellular and Molecular Bioengineering
Issue number4
StatePublished - Dec 2014

All Science Journal Classification (ASJC) codes

  • Modeling and Simulation
  • General Biochemistry, Genetics and Molecular Biology


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