Nucleic acid chromatography: Substitute solid support material for RPC-5 columns

J. M. Flanagan; R. K. Fujimura; K. Bruce Jacobson

doi:10.1016/0003-2697(86)90096-5

Nucleic acid chromatography: Substitute solid support material for RPC-5 columns

J. M. Flanagan, R. K. Fujimura, K. Bruce Jacobson

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.

Original language	English (US)
Pages (from-to)	299-304
Number of pages	6
Journal	Analytical Biochemistry
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(86)90096-5
State	Published - Mar 1986

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Nucleic acid chromatography: Substitute solid support material for RPC-5 columns",

abstract = "Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.",

author = "Flanagan, {J. M.} and Fujimura, {R. K.} and {Bruce Jacobson}, K.",

note = "Funding Information: Research was sponsored by the Office of Health and Environmental Research, U. S. Department of Energy under Contract DE-AC05-840R2 1400 with the Martin Marietta Energy Systems, Inc.",

year = "1986",

month = mar,

doi = "10.1016/0003-2697(86)90096-5",

language = "English (US)",

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pages = "299--304",

journal = "Analytical Biochemistry",

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T1 - Nucleic acid chromatography

T2 - Substitute solid support material for RPC-5 columns

AU - Flanagan, J. M.

AU - Fujimura, R. K.

AU - Bruce Jacobson, K.

N1 - Funding Information: Research was sponsored by the Office of Health and Environmental Research, U. S. Department of Energy under Contract DE-AC05-840R2 1400 with the Martin Marietta Energy Systems, Inc.

PY - 1986/3

Y1 - 1986/3

N2 - Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.

AB - Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.

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JO - Analytical Biochemistry

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ER -

Nucleic acid chromatography: Substitute solid support material for RPC-5 columns

Abstract

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