TY - JOUR
T1 - Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant
AU - Postle, Kathleen
AU - Nguyen, Toai T.
AU - Bertrand, Kevin P.
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Robert Sauer for his critical comments on repressor sequence homolo- gies, M. Kathleen Toth and Stephanie Broderick for their excellent technical assistance, and David Farrell for his contribution to the plasmid constructions. This work was supported by Research Fellowship GM06702 (K.P.), Research Grant AU6735 (K.P.B.), and Research Career Development Award AI00470 (K.P.B.) from the National Institutes of Health.
PY - 1984/6/25
Y1 - 1984/6/25
N2 - The Tn10 tetR gene encodes the repressor that regulates transcription of the Tnl0 tetracycline resistance determinant. We have determined the DNA sequence of the tetR gene and a 905 base pair region immediately 3′ to tetR. The tetR gene is located on a 701 base pair HincII restriction fragment. Deletions at either end of this region eliminate synthesis of the wild-type tetR protein in E. coli minicells, and eliminate tetR activity as measured by repression of β-galactosidase synthesis in tetA-lacZ operon fusion strains. Taken together, the DNA sequence and the genetic data indicate that tetR encodes a 207 amino acid protein with a calculated molecular weight of 23,328. This value is in good agreement with estimates of 23,000-25,000 based on electrophoretic mobility in SDS-polyaerylamide gels. There is 47% amino acid sequence homology between the deduced sequences of the Tn10 and RPl/Tnl721 tetR proteins. There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tnl0 tetR repressor and the DNA recognition regions of other DNA-binding proteins.
AB - The Tn10 tetR gene encodes the repressor that regulates transcription of the Tnl0 tetracycline resistance determinant. We have determined the DNA sequence of the tetR gene and a 905 base pair region immediately 3′ to tetR. The tetR gene is located on a 701 base pair HincII restriction fragment. Deletions at either end of this region eliminate synthesis of the wild-type tetR protein in E. coli minicells, and eliminate tetR activity as measured by repression of β-galactosidase synthesis in tetA-lacZ operon fusion strains. Taken together, the DNA sequence and the genetic data indicate that tetR encodes a 207 amino acid protein with a calculated molecular weight of 23,328. This value is in good agreement with estimates of 23,000-25,000 based on electrophoretic mobility in SDS-polyaerylamide gels. There is 47% amino acid sequence homology between the deduced sequences of the Tn10 and RPl/Tnl721 tetR proteins. There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tnl0 tetR repressor and the DNA recognition regions of other DNA-binding proteins.
UR - https://www.scopus.com/pages/publications/0021770671
UR - https://www.scopus.com/pages/publications/0021770671#tab=citedBy
U2 - 10.1093/nar/12.12.4849
DO - 10.1093/nar/12.12.4849
M3 - Article
C2 - 6330687
AN - SCOPUS:0021770671
SN - 0305-1048
VL - 12
SP - 4849
EP - 4863
JO - Nucleic acids research
JF - Nucleic acids research
IS - 12
ER -