On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells

Paula Beyer Hietpas; Andrew G. Ewing

doi:10.1080/10826079508014611

On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells

Paula Beyer Hietpas, Andrew G. Ewing

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 um Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.

Original language	English (US)
Pages (from-to)	3557-3576
Number of pages	20
Journal	Journal of Liquid Chromatography
Volume	18
Issue number	18-19
DOIs	https://doi.org/10.1080/10826079508014611
State	Published - Nov 1 1995

All Science Journal Classification (ASJC) codes

Molecular Medicine

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10.1080/10826079508014611

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title = "On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells",

abstract = "Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 um Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.",

author = "Hietpas, {Paula Beyer} and Ewing, {Andrew G.}",

note = "Funding Information: This research was supported in part by the National Institutes of Health. Funding Information: supported by the National Science Foundation.",

year = "1995",

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doi = "10.1080/10826079508014611",

language = "English (US)",

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journal = "Journal of Liquid Chromatography",

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TY - JOUR

T1 - On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells

AU - Hietpas, Paula Beyer

AU - Ewing, Andrew G.

N1 - Funding Information: This research was supported in part by the National Institutes of Health. Funding Information: supported by the National Science Foundation.

PY - 1995/11/1

Y1 - 1995/11/1

N2 - Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 um Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.

AB - Derivatization methods for analysis of single cell components by capillary electrophoresis with laser-induced fluorescence detection are reviewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell lines ranging in size from 8 to 140 um Pre-column, on-column, and post-column methods of derivatization are examined in order to determine which results in the least amount of sample handling and dilution leading to better detection limits for the analytes of interest.

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DO - 10.1080/10826079508014611

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EP - 3576

JO - Journal of Liquid Chromatography

JF - Journal of Liquid Chromatography

IS - 18-19

ER -

On-column and post-column derivatization for capillary electrophoresis with laser-induced fluorescence for the analysis of single cells

Abstract

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