TY - JOUR
T1 - Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries
AU - La Cour, Mette F.
AU - Mehrvar, Shima
AU - Kim, Joohyun
AU - Martin, Alicia
AU - Zimmerman, Michael A.
AU - Hong, Johnny C.
AU - Ranji, Mahsa
N1 - Publisher Copyright:
© 2017 Optical Society of America.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.
AB - Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.
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U2 - 10.1364/BOE.8.004419
DO - 10.1364/BOE.8.004419
M3 - Article
AN - SCOPUS:85031046226
SN - 2156-7085
VL - 8
SP - 4419
EP - 4426
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 10
M1 - #293196
ER -