TY - JOUR
T1 - Optimization of extracellular thermotolerant alkaline protease produced by marine Roseobacter sp. (MMD040)
AU - Shanmughapriya, S.
AU - Krishnaveni, J.
AU - Selvin, Joseph
AU - Gandhimathi, R.
AU - Arunkumar, M.
AU - Thangavelu, T.
AU - Kiran, G. Seghal
AU - Natarajaseenivasan, K.
N1 - Funding Information:
Fig. 9 Effects of different temperature on stability of the protease at 27°C (filled diamond), 30°C (filled square) 37°C (filled triangle), 40°C (crosses) and 50°C (open circle). Temperature stability of the purified enzyme was tested by preincubating at different temperatures for 1 h, the residual activity was measured by standard protease assay. The preincubated samples 0°C was used as a reference to calculate the residual activity. Data are presented as mean ± SD, n = 3 Acknowledgments JS is thankful to DST (SR/FT/L-19/2004), New Delhi and International Foundation for Science (RGA No. F/3776-1), Sweden for financial support. Authors are thankful to Dr. N. Tha-juddin, Head, Department of Microbiology for facilities and Dr. P.A. Thomas, Principal Scientist (Rtd), CMFRI, India for sponge identification.
PY - 2008/8
Y1 - 2008/8
N2 - Marine endosymbiontic Roseobacter sp. (MMD040), which produced high yields of protease, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in Luria-Bertani broth. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 37°C and 7.0, respectively. The enzyme exhibited maximum activity in pH range of 6-9 with an optimum pH of 8.0 and retained nearly 92.5% activity at pH 9.0. The enzyme was stable at 40°C and showed 89% activity at 50°C. Based on the present findings, the enzyme was characterized as thermotolerant alkaline protease, which can be developed for industrial applications.
AB - Marine endosymbiontic Roseobacter sp. (MMD040), which produced high yields of protease, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in Luria-Bertani broth. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 37°C and 7.0, respectively. The enzyme exhibited maximum activity in pH range of 6-9 with an optimum pH of 8.0 and retained nearly 92.5% activity at pH 9.0. The enzyme was stable at 40°C and showed 89% activity at 50°C. Based on the present findings, the enzyme was characterized as thermotolerant alkaline protease, which can be developed for industrial applications.
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U2 - 10.1007/s00449-007-0179-z
DO - 10.1007/s00449-007-0179-z
M3 - Article
C2 - 18071758
AN - SCOPUS:47349097365
SN - 1615-7591
VL - 31
SP - 427
EP - 433
JO - Bioprocess and biosystems engineering
JF - Bioprocess and biosystems engineering
IS - 5
ER -