Optimization of PCR template concentrations for in vitro transcription-translation reactions

Lisa Renshaw-Gegg; Mark J. Guiltinan

doi:10.1007/BF00130765

Optimization of PCR template concentrations for in vitro transcription-translation reactions

Lisa Renshaw-Gegg, Mark J. Guiltinan

Plant Science

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In vitro transcription-translation reactions can be used to rapidly and inexpensively synthesize small amounts of protein from cloned DNA sequences for biochemical studies. The use of PCR to amplify template sequences has many advantageous over conventional protocols. However, the amount of template needs to be carefully adjusted for optimal production of protein products.

Original language	English (US)
Pages (from-to)	679-682
Number of pages	4
Journal	Biotechnology Letters
Volume	18
Issue number	6
DOIs	https://doi.org/10.1007/BF00130765
State	Published - 1996

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF00130765

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title = "Optimization of PCR template concentrations for in vitro transcription-translation reactions",

abstract = "In vitro transcription-translation reactions can be used to rapidly and inexpensively synthesize small amounts of protein from cloned DNA sequences for biochemical studies. The use of PCR to amplify template sequences has many advantageous over conventional protocols. However, the amount of template needs to be carefully adjusted for optimal production of protein products.",

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AB - In vitro transcription-translation reactions can be used to rapidly and inexpensively synthesize small amounts of protein from cloned DNA sequences for biochemical studies. The use of PCR to amplify template sequences has many advantageous over conventional protocols. However, the amount of template needs to be carefully adjusted for optimal production of protein products.

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Optimization of PCR template concentrations for in vitro transcription-translation reactions

Abstract

All Science Journal Classification (ASJC) codes

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