Optimized over-expression of [FeFe] hydrogenases with high specific activity in Clostridium acetobutylicum

Gregory Von Abendroth, Sven Stripp, Alexey Silakov, Christian Croux, Philippe Soucaille, Laurence Girbal, Thomas Happe

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72 Scopus citations


It was previously shown that Clostridium acetobutylicum is capable to over-express various [FeFe] hydrogenases although the protein yield was low. In this study we report on doubling the yield of the clostridial hydrogenase by replacing the native gene hydA1Ca with a recombinant one via homologous recombination. The purified protein HydAlCa shows an unexpected high specific activity (up to 2257 μmol H2min -1 mg-1) for hydrogen evolution. Furthermore, the highly active green algal hydrogenase HydAlCr from Chlamydomonas reinhardtii was heterologously expressed in C. acetobutylicum, and purified with increased yield (1 mg protein per liter of cells) and high activity (625 nmol H 2 min-1mg-1). EPR studies demonstrate intact H-clusters for homologously and heterologously expressed [FeFe] hydrogenases in the CO-inhibited oxidized redox state, and prove the high efficiency of the C. acetobutylicum expression system.

Original languageEnglish (US)
Pages (from-to)6076-6081
Number of pages6
JournalInternational Journal of Hydrogen Energy
Issue number21
StatePublished - Nov 2008

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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