@article{0b835460f72b4a2781f29265b0089e09,
title = "Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells",
abstract = "Precise spatial and temporal regulation of dynamic morphogen signals during human development governs the processes of cell proliferation, migration, and differentiation to form organized tissues and organs. Tissue patterns spontaneously emerge in various human pluripotent stem cell (hPSC) models. However, the lack of molecular methods for precise control over signal dynamics limits the reproducible production of tissue patterns and a mechanistic understanding of self-organization. We recently implemented an optogenetic-based OptoWnt platform for light-controllable regulation of Wnt/β-catenin signaling in hPSCs for in vitro studies. Using engineered illumination devices to generate light patterns and thus precise spatiotemporal control over Wnt activation, here we triggered spatially organized transcriptional changes and mesoderm differentiation of hPSCs. In this way, the OptoWnt system enabled robust endothelial cell differentiation and cardiac tissue patterning in vitro. Our results demonstrate that spatiotemporal regulation of signaling pathways via synthetic OptoWnt enables instructive stem cell fate engineering and tissue patterning.",
author = "Hellwarth, {Peter B.} and Yun Chang and Arundhati Das and Liang, {Po Yu} and Xiaojun Lian and Repina, {Nicole A.} and Xiaoping Bao",
note = "Funding Information: We thank members of the Lian and Bao laboratories for technical assistance and critical reading of the manuscript. We are also gratefully acknowledge the Purdue Flow Cytometry and Cell Separation Facility. This study was supported by American Heart Association Postdoctoral Fellowship (18POST33990230 to Xiaoping Bao), Showalter Research Trust (Young Investigator Award to Xiaoping Bao), and startup funding from the Davidson School of Chemical Engineering and the College of Engineering at Purdue (Xiaoping Bao). Funding Information: We thank members of the Lian and Bao laboratories for technical assistance and critical reading of the manuscript. We are also gratefully acknowledge the Purdue Flow Cytometry and Cell Separation Facility. This study was supported by American Heart Association Postdoctoral Fellowship (18POST33990230 to Xiaoping Bao), Showalter Research Trust (Young Investigator Award to Xiaoping Bao), and startup funding from the Davidson School of Chemical Engineering and the College of Engineering at Purdue (Xiaoping Bao). Publisher Copyright: {\textcopyright} 2021 The Authors. Advanced Genetics published by Wiley Periodicals LLC.",
year = "2021",
month = sep,
doi = "10.1002/ggn2.202100011",
language = "English (US)",
volume = "2",
journal = "Advanced Genetics",
issn = "2641-6573",
publisher = "John Wiley & Sons Inc.",
number = "3",
}