Orai1 and STIM1 Are Critical for Breast Tumor Cell Migration and Metastasis

Shengyu Yang; J. Jillian Zhang; Xin Yun Huang

doi:10.1016/j.ccr.2008.12.019

Orai1 and STIM1 Are Critical for Breast Tumor Cell Migration and Metastasis

Shengyu Yang, J. Jillian Zhang, Xin Yun Huang

Research output: Contribution to journal › Article › peer-review

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Abstract

Tumor metastasis is the primary cause of death of cancer patients. Understanding the molecular mechanisms underlying tumor metastasis will provide potential drug targets. We report here that Orai1 and STIM1, both of which are involved in store-operated calcium entry, are essential for breast tumor cell migration in vitro and tumor metastasis in mice. Reduction of Orai1 or STIM1 by RNA interference in highly metastatic human breast cancer cells or treatment with a pharmacological inhibitor of store-operated calcium channels decreased tumor metastasis in animal models. Our data demonstrate a role for Orai1 and STIM1 in tumor metastasis and suggest store-operated calcium entry channels as potential cancer therapeutic targets.

Original language	English (US)
Pages (from-to)	124-134
Number of pages	11
Journal	Cancer Cell
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/j.ccr.2008.12.019
State	Published - Feb 3 2009

All Science Journal Classification (ASJC) codes

Oncology
Cell Biology
Cancer Research

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10.1016/j.ccr.2008.12.019

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title = "Orai1 and STIM1 Are Critical for Breast Tumor Cell Migration and Metastasis",

abstract = "Tumor metastasis is the primary cause of death of cancer patients. Understanding the molecular mechanisms underlying tumor metastasis will provide potential drug targets. We report here that Orai1 and STIM1, both of which are involved in store-operated calcium entry, are essential for breast tumor cell migration in vitro and tumor metastasis in mice. Reduction of Orai1 or STIM1 by RNA interference in highly metastatic human breast cancer cells or treatment with a pharmacological inhibitor of store-operated calcium channels decreased tumor metastasis in animal models. Our data demonstrate a role for Orai1 and STIM1 in tumor metastasis and suggest store-operated calcium entry channels as potential cancer therapeutic targets.",

author = "Shengyu Yang and Zhang, {J. Jillian} and Huang, {Xin Yun}",

note = "Funding Information: We thank D. Webb for the paxillin-GFP plasmid, J. Massagu{\'e} for MDA-MB-231 cells, V. Ponomarev for the TGL reporter plasmid, G. Gupta for help with bioluminescent imaging, and J. Hou for assistance with histological staining. We are grateful to T. Maack, L. Palmer, M. Zhu, and members of our laboratory for critical reading of the manuscript. This research was supported by the US Department of Defense Breast Cancer Research Program. S.Y. is a recipient of a Postdoctoral Fellowship in Breast Cancer Research from the New York State Department of Health. ",

year = "2009",

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doi = "10.1016/j.ccr.2008.12.019",

language = "English (US)",

volume = "15",

pages = "124--134",

journal = "Cancer Cell",

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T1 - Orai1 and STIM1 Are Critical for Breast Tumor Cell Migration and Metastasis

AU - Yang, Shengyu

AU - Zhang, J. Jillian

AU - Huang, Xin Yun

N1 - Funding Information: We thank D. Webb for the paxillin-GFP plasmid, J. Massagué for MDA-MB-231 cells, V. Ponomarev for the TGL reporter plasmid, G. Gupta for help with bioluminescent imaging, and J. Hou for assistance with histological staining. We are grateful to T. Maack, L. Palmer, M. Zhu, and members of our laboratory for critical reading of the manuscript. This research was supported by the US Department of Defense Breast Cancer Research Program. S.Y. is a recipient of a Postdoctoral Fellowship in Breast Cancer Research from the New York State Department of Health.

PY - 2009/2/3

Y1 - 2009/2/3

N2 - Tumor metastasis is the primary cause of death of cancer patients. Understanding the molecular mechanisms underlying tumor metastasis will provide potential drug targets. We report here that Orai1 and STIM1, both of which are involved in store-operated calcium entry, are essential for breast tumor cell migration in vitro and tumor metastasis in mice. Reduction of Orai1 or STIM1 by RNA interference in highly metastatic human breast cancer cells or treatment with a pharmacological inhibitor of store-operated calcium channels decreased tumor metastasis in animal models. Our data demonstrate a role for Orai1 and STIM1 in tumor metastasis and suggest store-operated calcium entry channels as potential cancer therapeutic targets.

AB - Tumor metastasis is the primary cause of death of cancer patients. Understanding the molecular mechanisms underlying tumor metastasis will provide potential drug targets. We report here that Orai1 and STIM1, both of which are involved in store-operated calcium entry, are essential for breast tumor cell migration in vitro and tumor metastasis in mice. Reduction of Orai1 or STIM1 by RNA interference in highly metastatic human breast cancer cells or treatment with a pharmacological inhibitor of store-operated calcium channels decreased tumor metastasis in animal models. Our data demonstrate a role for Orai1 and STIM1 in tumor metastasis and suggest store-operated calcium entry channels as potential cancer therapeutic targets.

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EP - 134

JO - Cancer Cell

JF - Cancer Cell

IS - 2

ER -

Orai1 and STIM1 Are Critical for Breast Tumor Cell Migration and Metastasis

Abstract

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