Osteoblast adhesion of breast cancer cells with scanned image microscopy

Chiaki Miyasaka, Bernhard R. Tittmann, Robyn R. Mercer, Andrea M. Mastro

Research output: Contribution to journalConference articlepeer-review


Breast cancer frequently metastasizes, or spreads, to the bone. Upon colonizing bone tissue, the cancer cells stimulate osteoclasts (cells that break bone down), resulting in large lesions in the bone. It seems that breast cancer cells also affect osteoblasts (cells that build new bone), in addition to osteoclasts. To test this hypothesis, conditioned medium was collected from a bone-metastatic breast cancer cell line, MDA-MB-231, and then cultured with an immature osteoblast cell line, MC3T3-E1. To fcharacterize cell adhesion, MC3T3-E1 osteoblasts were examined with a mechanical scanning acoustic reflection microscope (SAM). The cells were cultured with or without MDA-MB-231 conditioned medium for 2 days, and then assayed microscopically. The SAM indicated that MC3T3-E1 osteoblasts were firmly attached to their plastic substrate. However, MC3T3-E1 cells cultured with MDA-MB-231 conditioned medium displayed both an abnormal shape and poor adhesion at the substrate interface. These data taken together suggest that MDA-MB-231 breast cancer cells alter MC3T3-E1 adhesion. Using optical microscopic techniques, we were not able to observe these differences until the cells were quite confluent after 7 days of culture. However, using the SAM, we were able to detect these changes within 2 days of culture with MDA-MB-231 conditioned medium.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2004
EventHealth Monitoring and Smart Nondestructive Evaluation of Structural and Biological Systems III - San Diego, CA, United States
Duration: Mar 15 2004Mar 17 2004

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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